Description

• General description: Conductivity (1.0 M, 24°C): <100 µmhos.
• Application: CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
• Warning: Toxicity: Harmful (C)
• Reconstitution: Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.
• Other Notes: Chen, Y., et al. 1992. Biochemistry 31, 2415.Ofri, D., et al. 1992. J. Neurochem. 58, 628.Ransom, R.W., et al. 1992. Biochem. Pharmacol. 43, 1823.Simonds, W.F., et al. 1992. Proc. Natl. Acad. Sci. USA77, 4623.Yannariello-Brown, J., and Weigel, P.H. 1992. Biochemistry31, 576.Hjelmeland, L.M. 1980. Proc. Natl. Acad. Sci. USA77, 6368.Simonds, W.F., et al. 1980. Proc. Natl. Acad. Sci. USA77, 4623.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany