Description

• General description: Zwitterionic detergent ideal for solubilization of membrane proteins, analytical, diagnostic, chromatography, electrophoresis work, and enzymology and immunology applications.Suitable for Molecular BiologyConductivity (10%, water): ≤50 µmhoDNase: None detectedProtease: None detectedPurity (TLC): Single spotRNase: None detectedSolubility: 20% in water, passes test Intended for laboratory and manufacturing use only. Not for drug, food, or household use.
• Application: CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
• Legal Information: CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany