252859

Tris Base

Manufacturer: Sigma Aldrich

CAS Number: 77-86-1

Synonym(S): Tris(hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol

Select a Size

Pack Size SKU Availability Price
100 G 252859-100-G In Stock ₹ 2,380.00
500 G 252859-500-G In Stock ₹ 6,080.00

252859 - 100 G

₹ 2,380.00

In Stock

Quantity

1

Base Price: ₹ 2,380.00

GST (18%): ₹ 428.40

Total Price: ₹ 2,808.40

product name

Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%

grade

ACS reagent

Quality Level

200

Assay

≥99.8%99.8-100.1%

form

crystalline

technique(s)

affinity chromatography: suitable

impurities

≤0.005% insolubles≤2% water

color

white

useful pH range

7-9

pKa (25 °C)

8.1

Other Options

Image Product Name Manufacturer Price Range
NC0761128
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 100 g
Sigma Aldrich Fine Chemicals Biosciences ₹ 4,945.37
NC1971981
Santa Cruz Biotechnology Tris base | 77-86-1 | MFCD00004679 | 10 kg
Santa Cruz Biotechnology ₹ 57,085.63
50-184-6009
Sigma Aldrich Fine Chemicals Biosciences Tris(Hydroxymethyl)Aminomethane | 77-86-1 | MFCD00004679 | 500g
Sigma Aldrich Fine Chemicals Biosciences ₹ 14,883.16
NC0910431
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 1 kg
Sigma Aldrich Fine Chemicals Biosciences ₹ 18,865.98
50-184-6031
Sigma Aldrich Fine Chemicals Biosciences Trizma(R) base >=99.9% (titration), crystalline | 77-86-1 | MFCD00004679 | 1KG
Sigma Aldrich Fine Chemicals Biosciences ₹ 32,387.03
50-184-6022
Sigma Aldrich Fine Chemicals Biosciences Sigma 7-9(R) >=99% (titration), crystalline | 77-86-1 | MFCD00004679 | 100G
Sigma Aldrich Fine Chemicals Biosciences ₹ 4,457.68
50-164-7577
Indofine Chemical TRIS(HYDROXYMETHYL)AMINOMETHANE, ULTRAPURE >99.9%, Products for Life Science, 77-86-1, >99.9%, 500 gm
Indofine Chemical ₹ 8,953.85
NC1624731
Thermo Scientific Production Chemicals and Services Trometamol | 77-86-1 | 121.14 g/mol | 2.5-KG
Thermo Scientific Production Chemicals and Services --
NC1612642
Thermo Scientific Production Chemicals and Services Trometamol | 77-86-1 | 121.14 g/mol | 50-KG
Thermo Scientific Production Chemicals and Services ₹ 11,30,231.34
50-165-7245
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 250g
Sigma Aldrich Fine Chemicals Biosciences ₹ 7,760.29
...

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Description

  • General description: Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma base, plays a crucial role in diverse research applications as a biological buffer. It is extensively used in the formulation of buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), owing to its optimal pKa of 8.1, making it well-suited for maintaining pH within the physiological range (pH 7 -9) applicable to most living organisms. However, caution might be necessary when studying some proteins, as Tris has the potential to interfere with the activity of certain enzymes. Tris base can be employed both independently as a buffer and as a component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, and TBE buffer, among others. Importantly, Tris base is characterized by its purity, essential stability, and relative non-hygroscopic nature.In laboratory settings, Tris base is indispensable for the preparation of buffers compatible with biological fluids, acting as a standard pH solution. It facilitates various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it contributes to studies involving cell membrane permeability and buffer preparation.
  • Application: Tris(hydroxymethyl)aminomethane has been used: as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.[1][2][3]as a buffer in flow cell sample preparation for Raman spectroscopy[4]as a wash buffer in Western blotting[5]as a buffer component in decellularization of tissues[6]
  • Features and Benefits: Efficient buffering within the pH range of 7-9 with a pKa of 8.1 (25 °C)Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applicationsCan be used in Cell Biology, and Biochemical research
  • Other Notes: For additional information on our range of Biochemicals, please complete this form.
  • Legal Information: Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

SAFETY INFORMATION

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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product name:
Tris(hydroxymethyl)aminomethane, ACS reagent, ≥99.8%

grade:
ACS reagent

Quality Level:
200

Assay:
≥99.8%99.8-100.1%

form:
crystalline

technique(s):
affinity chromatography: suitable

impurities:
≤0.005% insolubles≤2% water

color:
white

useful pH range:
7-9

pKa (25 °C):
8.1

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