Description

• General description: Guanidine hydrochloride, or aminoformamidine hydrochloride, is a versatile chemical compound widely used in cell biology and biochemical research due to its potent chaotropic properties. Its ability to disrupt protein and nucleic acid structures makes it valuable for purifying proteins and nucleic acids, particularly mRNA. Guanidine hydrochloride unfolds proteins, facilitating their purification and refolding, and it is effective in solubilizing denatured insoluble proteins at higher concentrations.At lower concentrations, guanidine hydrochloride has the intriguing ability to promote the refolding of denatured proteins, aiding in protein renaturation studies. In RNA extraction, it acts as a strong denaturant, disrupting cell structures and ensuring the integrity of extracted RNA by inactivating RNA enzymes. Overall, guanidine hydrochloride′s denaturing and renaturing properties make it an essential reagent for various cell biology applications, including protein purification, nucleic acid isolation, and protein refolding studies.
• Application: Guanidine hydrochloride has been used:for lysing homogenized brain tissuein the preparation of incubation buffer for Ni-sepharose protein binding, purification, propionylation, and on-bead digestion to minimize nonspecific binding to the affinity resinin the preparation of solutions/extraction buffer to extract soluble protein from the human tissue
• Biochem/physiol Actions: Guanidine hydrochloride, an efficient chaotropic reagent, is involved in RNA isolation, globular protein denaturation, and protein refolding studies. This denaturant is appropriate for performing rapid and single-step affinity-purification mass spectrometry (AP-MS) experiments.
• Features and Benefits: Highly versatile surfactant for your cell biology and biochemical research
• Other Notes: For additional information on our range of Biochemicals, please complete this form.