Description

• General description: Pyridoxamine is a derivative of vitamin B6.[1] Pyridoxamine is a water-soluble vitamin.[2] Vitamin B6 derivatives contain a core pyridine ring. Pyridoxaminecontains an amino methyl group at pyridine′s 4′ position.[3]
• Application: Pyridoxamine-5′-phosphate is suitable:in the enzymatic synthesis of cytidine diphosphate-4-keto-3, 6-dideoxyglucose[4]as a substrate for pyridoxamine-5′-phosphate phosphatase activity assay[5]as a substrate to characterize pyridoxine-5′-phosphate oxidase[6][7]for the absorbance and fluorescence studies of properties of NaBH4-reduced glycogen phosphorylase b to probe the microenvironment of PMP residue[8]as a substrate for characterization of pyridoxamine-phosphate transaminase(s) from various species and tissues
• Biochem/physiol Actions: Pyridoxal 5′-phosphate can be converted to pyridoxamine-5′-phosphate in the presence of (S)-α-methylbenzylamine (MBA) as the amine donor by using pyruvate transaminase as a biocatalyst.[9] Pyridoxine 5′-phosphate and pyridoxamine 5′-phosphate are oxidized to pyridoxal 5′-phosphate (PLP) using flavin mononucleotide (FMN) as the immediate electron acceptor and oxygen as the ultimate electron acceptor by the E. coli enzyme pyridoxine (pyridoxamine) 5′-phosphate oxidase.[10]