Description

• General description: Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]
• Application: Triton™ X-100 has been used:for permeabilization of cells before techniques like indirect immunofluorescence and flow cytometry[3][4]As a component of lysis buffer used for homogenization of cells[3][4][5]As a component of blocking buffer in immunofluorescence staining[6]For the preparation of cells in double fluorescence staining[4]
• Biochem/physiol Actions: Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
• Features and Benefits: Suitable for Molecular Biology and Biochemical ResearchFree from DNase, RNase, NICKase and proteaseTested to confirm low levels of heavy metal contamination, ensuring suitability for various applications.High purity product for research applicationsTested for the presence of Anionic and Cationic traces
• Other Notes: For additional information on our range of Biochemicals, please complete this form.
• Legal Information: Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow