Description

• General description: Triton™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.[1][2]
• Application: Triton™ X-100 has been used:In immunohistochemistry for staining the Flat-mount retinas[3]Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay[4]To permeabilise cells during Immunofluorescent microscopic studies[5]As a positive control in LDH assay to determine the cell membrane integrity[6]For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure[7]For the preparation of outer membrane protein exctract[8]As a component of extraction buffer along with tris-HCl, NaCl, CaCl2, ZnCl2, Brij 35 for homogenization of mice lung cells[9]In the treatment of tissue sections for Immunofluorescence labeling[10]
• Biochem/physiol Actions: Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.
• Features and Benefits: Ideal for sensitive molecular biology research applicationsTested for DNase and RNaseNon-ionic nondenaturing detergentSuperior wetting agent and emulsifierHighly versatile surfactant
• Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
• Legal Information: Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow