Antifoam 204

Description

• General description: Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.
• Application: Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.Antifoam 204 has been used:in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain[1]in fed-batch fermentation[2]to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth[3]in the batch fermentation with mineral medium[4]
• Components: Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.
• Other Notes: Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.
• Preparation Note: For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.