Antifoam SE-15

Description

• General description: Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.
• Application: Antifoam SE-15 has been used: in the preparation of ramified rolling circle amplification (RAM) amplification buffer.[1]to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.[2]as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. [3]as a component of autoinduction expression culture. [4]as a component of cyclic adenosine monophosphate (cAMP) detection buffer. [5]
• Features and Benefits: Controls foaming in microbial culture flasks or fermentationsNon-toxic to the culture medium Can be pumped to fermenter on an as-needed basis
• Components: Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .
• Preparation Note: Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-dilutedsuspensions should be used immediately.