Tamm-Horsfall Glycoprotein

Description

• General description: Research area: Immuno and CKSTamm-Horsfall glycoprotein (THGP) also known as uromodulin is abundantly present in mammalian urine. It is highly expressed in the thick ascending limb of the Henle′s loop in the kidney.[1] This protein consists of three epidermal growth factor (EGF) domains and a zona pellucida-like domain. THGP is a member of the glycosylphosphatidylinositol (GPI) protein family and is attached to the cell membrane by a C-terminal GPI- anchor.[2] The THGP gene is located on the human chromosome at 16p12.3.[3]
• Application: Tamm-Horsfall Glycoprotein has been used in luciferase activity assay of immortalized proximal tubular cells and human primary proximal tubular epithelial cells.[4] It has also been used as a standard for calibration of uromodulin in urine samples.[5]
• Biochem/physiol Actions: Tamm-Horsfall glycoprotein (THGP) may play a role in antibacterial host defense, inflammatory kidney disease, and the urogenital tract.[1] It exhibits protective effects against kidney stone formation by reducing the calcium oxalate precipitation. Mutations in the THGP gene lead to familial juvenile hyperuricemic nephropathy (FJHN), glomerulocystic kidney disease (GCKD), and medullary cystic kidney disease type 2 (MCKD-2).[2]Tamm-Horsfall Protein (THP) mediates the formation of mucous coat of the bladder, thereby regulating the permeability of the ascending loop of Henle.[2] THP is known to adhere to Escherichia coli strains with type 131 and type S32 fimbriae, thereby blocking their interaction with the uroepithelium. Additionally, THP activates cells by directly signaling through Toll-like receptor 4 (TLR4). It has been demonstrated that THP stimulates the production of tumor necrosis factor-alpha (TNF-α) and tissue factor in monocytes through TLR4.[6]
• Physical form: Lyophillized from deionized water containing 0.02% sodium azide.
• Storage and Stability: Store +2-8°C for up to 3 months after date of receipt. DO NOT FREEZE.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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