Description

• General description: Doxorubicin hydrochloride (DOX) is an anthracycline antibiotic isolated from Streptomyces peucetius var. caesius.[1][2] The water soluble anti-cancer agent DOX[3] is a hydroxy derivative of daunorubicin.[4]Doxorubicin also leads to the generation of reactive oxygen species (ROS), which further damage DNA, proteins, and membranes. It exerts cytotoxic, antitumor, anticancer, and antineoplastic activity and finds application in cancer, metabolomics, cell biology, and biochemical research.[4]
• Application: Doxorubicin hydrochloride has been used:in cell viability assays[5]MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay[6]as a drug in polymeric PLGA-based microparticulate drug delivery[7]to develop doxorubicin-resistant HepG2 cells (HepG2-DR) and K562 cells (K562-DR)[8]
• Biochem/physiol Actions: Naturally fluorescent anthracycline antibiotic, anticancer drug. Doxorubicin is a substrate of MRP1 which was first cloned from a DOX-resistant lung cancer cell line. Fluorescent property has been exploited for the measurement of drug efflux pump activities as well as resolving the important question of intracellular localization of various multidrug resistance proteins and the role of subcellular organelles (Golgi and lysosome) in the sequestration of drugs and its implication in drug resistant phenotypes.
• Features and Benefits: High-quality antibiotic suitable for multiple research applicationsIdeal for Cell Biology, Metabolomics, and Biochemical research.
• Preparation Note: Soluble in water and in isotonic sodium chloride solution, slightly soluble in methanol. Practically insoluble in chloroform, ether, and in other organic solvents.
• Storage and Stability: Tightly closed. Dry. Keep in a well -ventilated place. Keep locked up or in an area accessible
• Other Notes: For additional information on our range of Biochemicals, please complete this form.