Description

• General description: Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes.
• Application: Deoxyribonucleic acid from human placenta has been used:to compare the efficiency of DNA quantification methods[1] as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines[2]as an internal control to estimate the degree of fragmentation of circulating DNA[3]for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11[4]to compare the 70-bp P5 exon sequence between DNA of different human and primate species[5]to calculate copy number of genes, relative to normal human tissue[6]in PCR reactions[7]
• Recommended products: Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes.