Description

• General description: High quality double-stranded template DNA isolated from the thymus of male and female calves.
• Application: Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels[1] and for DNA quantification by fluorescent assay.[2]
• Features and Benefits: • No nuclease activity detected after 16 h at 37 °C incubation• High quality template DNA
• Quantity: One mg DNA is approx. 20 A260 units.
• Preparation Note: Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.[3]
• Reconstitution: Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.