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D9905

Denhardt′s Solution

Name: Denhardt′s Solution | Sigma Aldrich | Chemikart
Brand: Chemikart

for Northern and Southern blotting, lyophilized powder

Manufacturer: Sigma Aldrich

Synonym(S): Denhardt′s Solution For Blotting Technique

Select a Size

Pack Size	SKU	Availability	Price
5 ML	D9905-5-ML	In Stock	₹ 12,037.40
5 X 5 ML	D9905-5-X-5-ML	In Stock	₹ 42,520.60

D9905 - 5 ML

₹ 12,037.40

In Stock

Quantity

Base Price: ₹ 12,037.40

GST (18%): ₹ 2,166.732

Total Price: ₹ 14,204.132

grade

for molecular biology

Quality Level

100

form

lyophilized powder

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

2-8°C

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Description

General description: Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Application: Denhardt′s Solution has been used:as a component in hybridization buffer for the hybridization of brain sections[1]in in-situ hybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) process[2]in the preparation of hybridization mixture[3]
Biochem/physiol Actions: Denhardt′s solution and gelatin-chrome alum can be used to treat chromosomes for G-banding with trypsin after in situ hybridization.[4]
Components: Each vial of Denhardt′s powder contains 50mg Bovine Serum Albumin (BSA), 50mg Ficoll, and 50mg Polyvinylpyrrolidone (PVP).
Preparation Note: To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at -20°C for up to two years.

SAFETY INFORMATION

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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2-8°C

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