Description

• Application: N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals[1]for cross-linking of nanofiber matrices with mouse lung extract[2]for activation of substrate XNA oligonucleotides[3]
• Biochem/physiol Actions: Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.