Description

• General description: Proligo′s DNA phosphoramidites lead to high-yield and high-quality oligonucleotides due to their high coupling efficiency. The deprotection step of automated oligonucleotide synthesis is integral to synthesis time and final product quality. The high coupling efficiency of Proligo′s DNA phosphoramidites leads to high-yield and high-quality oligonucleotides.Changing the dG protecting group to the dimethylformamidine (dmf) base protecting group enables rapid synthesis of high-purity, high-yieldoligonucleotide, thus increasing the efficiency of high-throughputproduction.Key Features of dG(dmf)dG(dmf) is deprotected faster than the conventional dG(ib): thedeprotection time in concentrated ammonia is reduced to 2 hours at55 °C or 1 hour at 65 °CThe dG(dmf)-monomer is especially suitable for G-rich sequences:incomplete deprotection is greatly reduced in comparison with theconventional dG(ib)-monomerdG(dmf)-phosphoramidite is as stable in solution as the standard dA(bz)-,dC(bz)- and dT-phosphoramidites dG(dmf)-phosphoramidite can directly substitute for dG(ib)-phosphoramidite No change is required in the reagents commonly used for DNA synthesis(except a low concentration iodine oxidizer i.e., 0.02 M in iodine, should beemployed)DMT-dG(dmf) Phosphoramidite is configured for ABI Synthesizers.