Description

• General description: Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.
• Application: Hydrocortisone is used for the following applications:Used in myogenic induction and identification[1] Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)[2]Used in adipogenic differentiation (one of the components in adipogenic medium)[3]Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components[4]The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone[5]Used in differentiation assays of melanoma spheroid cells[6]
• Biochem/physiol Actions: Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.
• Physical form: powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
• Reconstitution: To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.