γ-Glutamyltransferase (GGT) Activity Colorimetric Assay Kit

Description

• General description: γ-Glutamyltransferase (GGT, EC 2.3.2.2) is a membrane-bound protein that catalyzes the transfer of -glutamyl moieties to acceptor molecules such as amino acids or peptides. GGT plays a key role in the λ-glutamyl cycle, a critical pathway for glutathione homeostasis as well as the detoxification of xenobiotics. Serum GGT levels can be elevated in many pathophysiological conditions such as cardiovascular disease, chronic liver disease, and metabolic syndrome. Elevated serum levels of GGT can also be indicative of oxidative stress.[1]
• Application: γ-Glutamyltransferase (GGT) Activity Colorimetric Assay Kit has been used to assess the activity of γ-glutamyltransferase.[2][3]
• Features and Benefits: Compatible with high-throughput handling systems.
• Suitability: Suitable for the measurement of gamma-glutamyl transferase (GGT) in a variety of samples including tissue, cells and serum
• Principle: The GGT Activity Colorimetric Assay kit provides a simple and direct procedure for measuring GGT activity in a variety of samples. GGT activity is determined by a coupled enzyme assay, in which the GGT transfers the γ-glutamyl group from the substrate L-γ-Glutamyl-p-nitroanilide, liberating the chromogen p-nitroanilide (pNA, 418 nm) proportional to the GGT present. One unit of GGT is the amount of enzyme that will generate 1.0μmole of pNA per minute at 37 °C.