Multidrug Resistance Assay Kit

Description

• General description: Acquired resistance to chemotherapy drugs, multidrug resistance or MDR, is a major contributor to treatment failure for many types of cancers.[1][2] MDR is typically associated with the increased expression of two ATP-dependent drug efflux pumps, P-Glycoprotein (P-gp or MDR1) and the Multidrug Resistance-Associated Protein (MRP1). These pumps actively expel chemotherapeutic agents, typically hydrophobic amphipathic natural products, from the cytoplasm to exterior of the cell.[3]This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λex = 490/λem = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.
• Features and Benefits: Compatible with high-throughput handling systems.
• Suitability: This kit is suitable for the screening of MDR pump inhibitors or for identifying cell lines with high MDR activity.
• Principle: This kit is utilizes a hydrophobic fluorescent dye molecule to assess MDR activity in cells. This dye rapidly penetrates cell membranes and becomes trapped resulting in an increase in fluorescence intensity (λEx = 490/λEm = 525 nm). In cells expressing MDR transporters, the dye is rapidly extruded by the transporters, resulting in decreased fluorescence intensity.