MAK173

Universal Fluorometric Kinase Assay Kit

sufficient for 250 fluorometric tests

Manufacturer: Sigma Aldrich

Synonym(S): Universal Kinase Test Kit

Select a Size

Pack Size SKU Availability Price
1 KIT MAK173-1-KIT In Stock ₹ 39,094.20

MAK173 - 1 KIT

₹ 39,094.20

In Stock

Quantity

1

Base Price: ₹ 39,094.20

GST (18%): ₹ 7,036.956

Total Price: ₹ 46,131.156

usage

sufficient for 250 fluorometric tests

detection method

fluorometric

relevant disease(s)

endocrinological disorders, diabetes; neurological disorders; cardiovascular diseases; cancer

storage temp.

−20°C

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Description

  • General description: Protein kinases are the enzymes that catalyze the transfer of phosphate group from a phosphate donor to an acceptor in a substrate protein. Kinases are of great interest to researchers due to their role in cell signaling and their roles in disease states. Most of the commercial protein kinase assay kits are based on monitoring either phosphopeptide formation or the ATP depletion. Kinase assays that rely on the detection of phosphopeptides require significant effort to identify an optimized peptide for the specific kinase of interest. The ATP depletion method suffers various interferences due to inhibition or activation of luciferase by compounds found in the biological matrix.
  • Suitability: Suitable for the determination of kinase activity and for screening and identifying kinase inhibitors
  • Principle: This Universal Fluorimetric Kinase Assay Kit is based on monitoring ADP formation, which is directly proportional to kinase activity. This enzyme-coupled kit provides a fast, efficient, and homogeneous assay to measure kinase activities. This assay kit is a non-radioactive and non-antibody based method to detect the amount of ADP produced from enzyme reaction without a separation step.This kit demonstrates high sensitivity (<0.3 μM ADP) and broad ATP tolerance (1-300 μM. This kit can be utilized for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors.

SAFETY INFORMATION

Pictograms

GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

WGK

WGK 3

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