MAK306

LCFC-LCAT Acyltransferase Activity Assay

Supplied by Roar Biomedical, Inc., sufficient for 100 fluorometric tests

Manufacturer: Sigma Aldrich

Select a Size

Pack Size SKU Availability Price
1 KIT MAK306-1-KIT In Stock ₹ 63,281.10

MAK306 - 1 KIT

₹ 63,281.10

In Stock

Quantity

1

Base Price: ₹ 63,281.10

GST (18%): ₹ 11,390.598

Total Price: ₹ 74,671.698

usage

sufficient for 100 fluorometric tests

application(s)

pharmaceutical

detection method

fluorometric

relevant disease(s)

cardiovascular diseases

storage temp.

2-8°C

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Description

  • General description: The plasma protein lecithin:cholesterol acyltransferase (LCAT) catalyzes the transfer of an acyl group from the sn2 position of phosphatidylcholine to the 3-hydroxyl group of cholesterol forming cholesteryl ester. This activity occurs on the surface of high density lipoprotein (HDL) and the cholesteryl esters formed by LCAT are incorporated into the core of HDL.
  • Features and Benefits: Non-enzymatic determination of cholesteryl ester formation. Not affected by LCAT inhibitors or other chemical compounds. Dilution or non-dilution type assay. Assay components stable for up to one year.Compatible with high-throughput handling systems.
  • Suitability: Suitable for high-throughput screening, mechanism of action studies and structureactivity relationship (SAR) work of LCAT in plasma and serum
  • Principle: The LCFC-LCAT Acyltransferase Activity Assay is a fluorometric assay useful for measuring the acyltransferase activity of LCAT in serum or plasma. The method detects changes in LCAT free cholesterol (LCFC) levels in the sample without the use of cholesterol oxidase, peroxidase, or the generation of hydrogen peroxide. Detection is not affected by iodoacetate or other LCAT inhibitors. LCAT activity results in a fluorometric (λEx = 320 nm/λEm = 405 nm) product proportional to the amount of free cholesterol present.

SAFETY INFORMATION

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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MAK306

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usage:
sufficient for 100 fluorometric tests

application(s):
pharmaceutical

detection method:
fluorometric

relevant disease(s):
cardiovascular diseases

storage temp.:
2-8°C

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