MAK318

α−Mannosidase Assay Kit

sufficient for 100 colorimetric tests

Manufacturer: Sigma Aldrich

Select a Size

Pack Size SKU Availability Price
1 KIT MAK318-1-KIT In Stock ₹ 37,418.10

MAK318 - 1 KIT

₹ 37,418.10

In Stock

Quantity

1

Base Price: ₹ 37,418.10

GST (18%): ₹ 6,735.258

Total Price: ₹ 44,153.358

detection method

colorimetric

relevant disease(s)

neurological disorders

storage temp.

2-8°C

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Description

  • General description: α-Mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children.
  • Features and Benefits: Compatible with high-throughput handling systems.
  • Suitability: Suitable for determining α-Mannosidase (AMA) activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media.
  • Principle: The α-Mannosidase Activity Assay Kit provides a simple and high-throughput adaptable assay for determining α-Mannosidase activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media. In this assay 4-nitrophenol is cleaved from a synthetic substrate. Nitrophenol becomes intensely colored with the addition of the stop reagent, producing a colorimetric (405 nm) result, directly proportional to the enzyme activity in the sample.One unit of AMA is the amount of enzyme that will convert 1.0 μmole of 4-Nitrophenyl-α-D-mannopyranoside to 4-Nitrophenol and α-D-Mannose per minute at 25 °C and pH 4.5. The kit has a linear detection range of 1–250 unit/L for a 10 minute reaction in a 96 well format.

SAFETY INFORMATION

Pictograms

GHS07

Signal Word

Warning

Hazard Statements

H319

Precautionary Statements

P264 - P280 - P305 + P351 + P338 - P337 + P313

Hazard Classifications

Eye Irrit. 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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