Chloride Assay Kit

Description

• General description: Chloride is the major extracellular anion in human body fluids. Chloride plays a key role in maintaining proper water distribution, osmotic pressure and electrolyte balance. Low chloride concentrations may be found with prolonged vomiting, extensive burns, metabolic acidosis, Addisonia crisis and renal diseases. Elevated chloride concentrations are associated with dehydration, congestive heart failure, hyperventilation and urinary obstructions. Determination of chloride in sweat is useful in diagnosing cystic fibrosis.
• Features and Benefits: Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM)Compatible with high-throughput handling systems.The optimized formulation has greatly enhanced reagent and signal stability.Low interference in biological samples, no pretreatments are needed. Assays can be directly performed on raw biological samples in the presence of lipid, protein and minerals such as magnesium, iron and zinc.
• Suitability: The kit is suitable for chloride determination in serum, plasma, urine, saliva, sweat, water, soil, food, and beverages, as well as for studying the effects of drugs on chloride metabolism.
• Principle: The Chloride Assay Kit is designed to measure chloride directly in biological samples without any pretreatment. The improved Fried method utilizes mercuric 2,4,6-tripyridyl-s-triazine, which forms a colored complex specifically with chloride. The intensity of the color, measured at 610 nm, is directly proportional to the chloride concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.