Description

• Application: Streptolysin O is a toxin secreted by Streptococcus pyogenes and is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery[1]. It is a potential anticancer agent and is used to study suicide cancer gene therapy[1].
• Biochem/physiol Actions: Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membrane to make a large pore (25 to 30 nm), which DNA, RNA, peptides and proteins may pass[1]. It is thiol-activated. It is inhibited by allicin, an active component of garlic[2].
• Packaging: 25KU
• Unit Definition: One unit will cause 50% lysis of 2% red blood cell suspensions in phosphate buffered saline, pH 7.4, after incubation at 37 °C for 30 min.
• Physical form: Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.
• Other Notes: Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place