CpGenome Universal Unmethylated DNA

Description

• General description: CpGenome Universal Unmethylated DNA Set provides 2 separate genomic DNA controls for gene methylation studies. This product is intended for use with the CpGenome DNA Modification Kit (S7820) and can be subsequently used as an unmethylated DNA control for gene methylation studies. Materials Provided: Universal Unmethylated DNA Vial A contains 5 micrograms (50μL) of human genomic DNA at a concentration of 0.1 μg/μL. Universal Unmethylated DNA Vial B contains 5 micrograms (50μL), of genomic DNA isolated from a primary human fetal cell line at a concentration of 0.1 μg/μL. Note: The DNA must first be bisulfite modified using (S7820) to use a specific primer set for the unmethylated form of the gene of interest. Validation: Methylation-specific PCR (MSP) was performed on the DNA following bisulfite modification. Various primer sets from the CpG WIZ product line were used in the assay. DNA from Vial A has been validated with primers from several CpG WIZ amplification kits. Vial B DNA is also unmethylated at several promoters assayed with the CpG WIZ kits and is supplied as an alternative source of DNA for promoters that may be partially methylated in Vial A DNA. CpG Genome and CpG WIZ Methylation Products apply technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered by U.S. Patent # 5,786,146.
• Packaging: 2 x 5 μg
• Physical form: Liquid in TE (10mM Tris-HCL, 0.1mM EDTA) with no preservatives.
• Storage and Stability: Recommended Storage: -15°C to -25°C, in aliquots, protected from freeze-thaws for up to two years from date of receipt. Do not freeze and thaw the same -20°C aliquot more than 3X for best results. Multiple freeze thaws can fragment DNA. Storage at -70°C can be used for longer term storage if necessary; minimize multiple freeze thaws for best results.
• Legal Information: CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.