Description

• General description: Agarose is commonly used in research for gel electrophoresis to separate DNA and RNA fragments. It is a natural polysaccharide isolated and purified from red algae. It has a linear structure consisting of alternating D-galactose and 3,6-anhydro-L-galactose units.[1] When dissolved in solution, upon cooling, it forms a solid yet porous gel to use as a medium to separate nucleic acid fragments by size. When an electrical current is applied the charged molecules migrate through the gel.
• Application: Agarose tablets can be used in research applications for:Separation and analysis of nucleic acids by agarose gel electrophoresis, Northern Blot, or Southern Blot[2]Single Cell Gel Electrophoresis (SCGE), also known as comet assaysHydrocolloid gels to study the structural influence of gels on the release of carbohydrates[3]Ouchterlony double immunodiffusion plates used to quantify antibodies and antigensRadial immunodiffusion (RID)
• Features and Benefits: Qualified for molecular biology applications Free of endonucleases: DNase and RNaseConvenient, quick and easy to useLow EEO and gelling temperature for routine analysisHigh gel strength 1,200 g/cm2 (1% gel)Separates DNA & RNA fragments 100bp - 23kb in 2% - 0.5% agarose gel, respectively.Northern or Southern blotting, preferred for fragment sizes >1kb