IROA TruQuant Yeast Extract Workflow Kit

Description

• Application: TruQuant IQQ is a high-quality quantitation system for making simultaneous accurate biological measurements on several hundred biochemicals in small quantities of biological samples. This is achieved by (1) spiking a complex Internal Standard (WORKFLOW-A) into a biological sample to a) quantify all the biochemicals in the sample relative to their counterparts in the Internal Standard, b) suppression-correct each compound and c) normalize sample to sample variances; and (2) injecting the same well characterized Long-Term Reference Standard (WORKFLOW-B) to create a daily retention time (RT) library of all compounds to be found in the Internal Standard for reproducible ID, and to measure day-to-day (QA/QC) to assure reproducible instrument performance. The system is completely automated using IROA ClusterFinder™ software.IROA TruQuant IQQ Workflow Kit contains the materials and tools for the analysis of 90 experimental samples. The kit is intended to be used for mass spectrometry metabolomics applications.
• Features and Benefits: Identification: Confidence in identifying common and new metabolitesQuantification: Comprehensive correction of analytical and biological variability for all analytesQuality Control: Convenient and automated tools for assuring reproducibility within/across studiesSimultaneous metabolite identification, quantitation and platform QCAccurate compound formula ID for MS alone; complete ID with addition of SWATH, or IMIROA patterns discriminate peaks of biological origin from artefactual peaks allowing the removal of false dataIROA-based fragments have the IROA ratio pattern of their parent peaks and can be identified as such using the “peak correlation” ClusterFinder™ moduleRetention Index within the sample allows “unknowns” to be tracked with precisionSuppression-corrected measurements for significantly better quantitationNormalizes (suppression-corrected) experimental samples to overcome day-to-day, instrument-to-instrument variancesClusterFinder™ software solution builds libraries, IDs/quantitates compounds and normalizes dataEnsures high level QC for accurate and reproducible results
• Other Notes: ClusterFinder™ software package is distributed with and is tailored to work with the IROA TruQuant IQQ kit. A user manual and video instructions are provided.To download the software package, click here - ClusterFinder software.Link to the ClusterFinder™ Videos below:IROA Peaks BasicsCF Process OverviewCF InitiationCF New Project & Add DataCF Unbiased Search for IROA peaksCF Libraries & MethodsCF BinTree - bins are displayed as a treeCF Curation 1: Untargeted - quality of the peaksCF Curation 2: CascadeTo access the MS Calculator Cascade Spreadsheet, follow the link below:MS Calculator
• Legal Information: ClusterFinder is a trademark of IROA Technologies LLC