Description

• General description: SMPT is a heterobifunctional sulfhydryl- and amine-reactive cross-linker. SMPT is used often for conjugating a toxin molecule to a monoclonal antibody directed against a cell-surface antigen. These immunotoxins produced with SMPT are highly potent as the cleavable disulfide imparts increased cytotoxicity compared to conjugates without a cleavable bond, such as those produced with SPDP. Also, next to the pyridine-2-thione in the spacer arm are a benzene ring and a methyl group adjacent to a carbon that hinders the disulfide bond, allowing exceptional conjugate stability in vivo. SMPT has the added benefit of being more stable to hydrolysis in aqueous solutions than other NHS cross-linkers.
• Features and Benefits: Reactive groups: NHS ester and pyridyldithiolReactive towards: amino and sulfhydryl groupsCleavable with disulfide reducing agentsWater-insoluble (dissolve first in DMF or DMSO)Contains a hindered disulfide bond; has formed immunotoxins with improved stabilityIn vitro, an SMPT conjugate was as effective as conjugates formed with SPDP and 2-ITCompare to other varieties of SPDP-type reagents, including pegylated forms
• Caution: This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.