Anti-SDHD/CybS Antibody, clone 1F5-H2L2 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1F5-H2L2 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects Succinate dehydrogenase complex subunit D (SDHD). It targets an epitope within 12 amino acids from the Internal region of the protein.
• Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids from the Internal region of human Succinate dehydrogenase complex subunit D.
• Application: Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SDHD/CybS protein in HeLa cell lysate.Tested ApplicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SDHD/CybS in lysates from HepG2, COS-7, and NIH/3T3 cells.Flow Cytometry Analysis: 0.1 µg from a representative lot detected SDHD/CybS in one million HeLa cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SDHD/CybS in human kidney tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SDHD/CybS in NIH/3T3 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
• Target description: Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (CybS)(UniProt: O14521; also known as CII-4, QPs3, Succinate dehydrogenase complex subunit D, Succinate-ubiquinone oxidoreductase cytochrome b small subunit, Succinate-ubiquinone reductase membrane anchor subunit) is encoded by the SDHD (also known as SDH4 ) gene (Gene ID: 6392) in human. It is a multi-pass membrane protein and primarily localized in the inner motochondrian membrane. SDHD is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SDH). The specific role of SDH is to transfer electrons from succinate to ubiquinone, and the SDHD subunit is involved in anchoring the SDH complex to the inner mitochondrial membrane. SDHD is expressed in various tissues, with particularly high expression in tissues with high energy demands. Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumorsuppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth. Defective SDHD has been linked to Carney-Stratakis syndrome, a condition characterized by concurrent paraganglioma and gastric stromal sarcomas. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.