Anti-FIS1 Antibody, clone 1C7 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1C7 is a ZooMAb® rabbit recombinant monoclonal antibody that detects FIS1. It targets an epitope within 24 amino acids from the N-terminal cytoplasmic domain.
• Immunogen: KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal, cytoplasmic domain of human FIS1.
• Application: Quality Control Testing Evaluated by Western Blotting in HeLa cell lysate.Western Blotting: A 1:10,000 dilution of this antibody detected FIS1 in HeLa cell lysate.Tested Applications Western Blotting Analysis: 1:1,000-10,000 dilutions from a representative lot detected FIS1 in lysates from Raji cells and mouse liver tissue.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FIS1 in human kidney tissue sections.Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected FIS1 in HeLa cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Mitochondrial fission 1 protein (UniProt: Q9Y3D6; also known as FIS1 homolog (hFis1), Tetratricopeptide repeat protein 11 (TPR repeat protein 11)) is encoded by the FIS1 (also known as TTC11, CGI-135) gene (Gene ID: 51024) in human. FIS1 is a small, integral mitochondrial outer membrane protein characterized by a tetratricopeptide repeat (TPR) domain which facilitates protein-protein interactions. The cytosolic domain interacts with other proteins involved in mitochondrial fission. It acts by recruiting cytosolic factors such as dynamin-related protein 1 (DRP1), facilitating the assembly of the fission machinery that constricts and divides mitochondria. This process is crucial for mitochondrial distribution and health, affecting cellular energy production and apoptosis. Dysregulation of FIS1 expression or activity is associated with neurodegenerative diseases such as Alzheimer′s and Parkinson′s, where impaired mitochondrial dynamics contribute to cellular dysfunction and death. Additionally, FIS1 has been linked to metabolic disorders, as proper mitochondrial function is crucial for energy balance and metabolism. In cancer, altered mitochondrial fission can affect apoptosis and cellular proliferation, implicating FIS1 in tumorigenesis and cancer progression. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.