7089810
TSKgel™ Chelate-5PW HPLC Column, Sold by MilliporeSigma™ Supelco™
Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, loaded with divalent metal ions by chelation.
Manufacturer: Fischer Scientific
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pH
2 to 12
Temperature
≤45°C
Product Line
TSKgel
Technique
Affinity
Solvents
≤ 20% Organic Solvent
Stationary Phase
Iminodiacetic Acid
Matrix
Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer
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Description
- High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins Utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+ Column is loaded with divalent metal ions by chelation Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at a neutral pH Retained compounds can be eluted with buffer containing imidazole or glycine A decreasing pH gradient can be used for elution purposes as well For successful use of this retention mechanism, select the proper metal ions for chelation and an elution buffer to desorb the analytes Applications: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides
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pH: 2 to 12
Temperature: ≤45°C
Product Line: TSKgel
Technique: Affinity
Solvents: ≤ 20% Organic Solvent
Stationary Phase: Iminodiacetic Acid
Matrix: Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer
pH:
2 to 12
Temperature:
≤45°C
Product Line:
TSKgel
Technique:
Affinity
Solvents:
≤ 20% Organic Solvent
Stationary Phase:
Iminodiacetic Acid
Matrix:
Iminodiacetic Acid Phase/Hydroxylated Methacrylic Polymer
pH: 2 to 12
Temperature: 10°C to 45°C
Product Line: TSKgel
Technique: __
Solvents: ≤ 20% Organic Solvent
Stationary Phase: DEAE (diethylaminoethyl)
Matrix: DEAE (diethylaminoethyl) Phase/Hydroxylated Methacrylic Polymer
pH:
2 to 12
Temperature:
10°C to 45°C
Product Line:
TSKgel
Technique:
__
Solvents:
≤ 20% Organic Solvent
Stationary Phase:
DEAE (diethylaminoethyl)
Matrix:
DEAE (diethylaminoethyl) Phase/Hydroxylated Methacrylic Polymer
pH: 2 to 12
Temperature: 10°C to 45°C
Product Line: TSKgel
Technique: Cation Exchange
Solvents: ≤ 20% Organic Solvent
Stationary Phase: Sulfonic Acid
Matrix: Sulfonic Acid Phase/Hydroxylated Methacrylic Polymer
pH:
2 to 12
Temperature:
10°C to 45°C
Product Line:
TSKgel
Technique:
Cation Exchange
Solvents:
≤ 20% Organic Solvent
Stationary Phase:
Sulfonic Acid
Matrix:
Sulfonic Acid Phase/Hydroxylated Methacrylic Polymer
pH: 2 to 12
Temperature: 10°C to 50°C
Product Line: TSKgel
Technique: HIC HPLC
Solvents: ≤ 50% Organic Solvent
Stationary Phase: Phenyl
Matrix: Phenyl Phase/Hydroxylated Methacrylic Polymer
pH:
2 to 12
Temperature:
10°C to 50°C
Product Line:
TSKgel
Technique:
HIC HPLC
Solvents:
≤ 50% Organic Solvent
Stationary Phase:
Phenyl
Matrix:
Phenyl Phase/Hydroxylated Methacrylic Polymer