7089814
TSKgel™ Boronate-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™
High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins.
Manufacturer: Fischer Scientific
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Flow Rate
1.2 mL/min
Particle Size
10 μm
Solvents
≤ 20% Organic Solvent
Stationary Phase
M-Aminophenyl Boronate
Sample Type
Proteins
Technique
Affinity
Max. Pressure
30 bar
pH
2 to 9
Temperature
>0°C
Product Line
TSKgel
Matrix
M-Aminophenyl Boronate Phase/Hydroxylated Methacrylic Polymer
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Description
- High efficiency, resin-based affinity columns separate or purify many enzymes and other proteins Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds, and catecholamines Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers Adsorption onto the matrix takes place in basic buffers such as HEPES and morpholine Desorption takes place in carbohydrate or amine-containing mobile phases such as sorbitol or Tris Resin Adsorption Capacity: 40 μmol/mL
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Flow Rate: 1.2 mL/min
Particle Size: 10 μm
Solvents: ≤ 20% Organic Solvent
Stationary Phase: M-Aminophenyl Boronate
Sample Type: Proteins
Technique: Affinity
Max. Pressure: 30 bar
pH: 2 to 9
Temperature: >0°C
Product Line: TSKgel
Matrix: M-Aminophenyl Boronate Phase/Hydroxylated Methacrylic Polymer
Flow Rate:
1.2 mL/min
Particle Size:
10 μm
Solvents:
≤ 20% Organic Solvent
Stationary Phase:
M-Aminophenyl Boronate
Sample Type:
Proteins
Technique:
Affinity
Max. Pressure:
30 bar
pH:
2 to 9
Temperature:
>0°C
Product Line:
TSKgel
Matrix:
M-Aminophenyl Boronate Phase/Hydroxylated Methacrylic Polymer
Flow Rate: __
Particle Size: 5 μm
Solvents: 0-100% Organic Solvent
Stationary Phase: Carbamoyl
Sample Type: __
Technique: HILIC
Max. Pressure: __
pH: 2 to 7.5
Temperature: 10°C to 80°C
Product Line: TSKgel
Matrix: Carbamoyl Phase/Silica Particle Platform
Flow Rate:
__
Particle Size:
5 μm
Solvents:
0-100% Organic Solvent
Stationary Phase:
Carbamoyl
Sample Type:
__
Technique:
HILIC
Max. Pressure:
__
pH:
2 to 7.5
Temperature:
10°C to 80°C
Product Line:
TSKgel
Matrix:
Carbamoyl Phase/Silica Particle Platform
Flow Rate: __
Particle Size: 7 μm
Solvents: 0-100% Organic Solvent
Stationary Phase: C18 (octadecyl)
Sample Type: __
Technique: __
Max. Pressure: __
pH: 2 to 12
Temperature: 5°C to 50°C
Product Line: TSKgel
Matrix: C18 (octadecyl) Phase/Polymer Particle Platform
Flow Rate:
__
Particle Size:
7 μm
Solvents:
0-100% Organic Solvent
Stationary Phase:
C18 (octadecyl)
Sample Type:
__
Technique:
__
Max. Pressure:
__
pH:
2 to 12
Temperature:
5°C to 50°C
Product Line:
TSKgel
Matrix:
C18 (octadecyl) Phase/Polymer Particle Platform
Flow Rate: __
Particle Size: 2.7 μm
Solvents: __
Stationary Phase: C30 (triacontyldimethyl)
Sample Type: __
Technique: Fused-Core HPLC
Max. Pressure: __
pH: 2 to 9
Temperature: __
Product Line: Ascentis
Matrix: C30 (triacontyldimethyl) Phase/Fused-Core™ Particle Platform
Flow Rate:
__
Particle Size:
2.7 μm
Solvents:
__
Stationary Phase:
C30 (triacontyldimethyl)
Sample Type:
__
Technique:
Fused-Core HPLC
Max. Pressure:
__
pH:
2 to 9
Temperature:
__
Product Line:
Ascentis
Matrix:
C30 (triacontyldimethyl) Phase/Fused-Core™ Particle Platform