Thermo Scientific™ DNAPac™ RP Columns

Description

• Achieve superior reversed phase oligonucleotide separations using the Thermo Scientific™ DNAPac™ RP HPLC columns
• The unique chemistry is designed for analysis of oligonucleotides and double-stranded (ds) DNA/RNA fragments using LC-UV or LC-MS
• The column chemistry provides excellent performance under a broad range of pH, temperature, and mobile phase compositions
• In addition, the wide pore size of the resin provides excellent separation of large double-stranded nucleic acids up to 10k base pairs
• High Performance Reversed-Phase LC Columns The DNAPac RP column is based on spherical wide-pore 4μm polymer resin
• The phase is highly stable to extremes of pH (0-14), and temperature (5-110°C), and operates with mobile phases compatible with direct mass spectrometry
• The column may be operated in both HPLC and UHPLC instruments, producing high resolution at high throughput for oligonucleotides and resolves both small and very large dsDNA segments
• DNAPac RP reversed phase (RP) column is specifically designed for separation of oligonucleotides and DNA/RNA fragments
• The stationary phase is fully compatible with mass spectrometry friendly organic solvents and ion-pair reagents such as triethylamine (TEA) and hexylamine (HA)
• The wide pore size of polymer resin enables efficient separation of large nucleic acids with low carry-over
• Columns designed for reversed phase oligonucleotide LC and LC/MS separations Designed for ion-pair reversed-phase (IP-RP) separations of both oligonucleotides and double-stranded nucleic acids High resolution and high throughput Excellent MS compatibility Wide operating pH range (0-14) and high temperature stability (up to 110°C)