18-374-058

Invitrogen™ 5' RACE System for Rapid Amplification of cDNA Ends, version 2.0

Manufacturer: Invitrogen™

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Pack Size SKU Availability Price
Each of 1 18-374-058-Each-of-1 In Stock ₹ 1,10,543.52

18-374-058 - Each of 1

₹ 1,10,543.52

In Stock

Quantity

1

Base Price: ₹ 1,10,543.52

GST (18%): ₹ 19,897.834

Total Price: ₹ 1,30,441.354

Product Type

cDNA library

Content And Storage

The 5´ RACE System is supplied with the components listed in Table 1. Store the reagents at -20°C and the DNA purification components at +4°C.

For Use With (Application)

cDNA Libraries & Library Construction

Quantity

10 Reactions

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Description

  • The 5' RACE System provides a set of prequalified reagents intended for synthesis of first-strand cDNA, purification of first-strand products, homopolymeric tailing, and preparation of target cDNA for subsequent amplification by PCR
  • Control RNA, DNA, and primers are provided for monitoring system performance
  • The system is useful for the amplification of rare messages for which little sequence information may be available, and for capturing the 5' end information of mRNA
  • Uses advanced capabilities of SuperScriptII RNase H- RT for greater first-strand cDNA yields and increased cDNA lengths Prepares specific cDNA from up to 1μg of total or poly(A)+ RNA in one reaction Offers improved first-strand cDNA purification procedure that yields cDNA suitable for tailing Uses recombinant TdT with optimized reaction conditions in PCR-compatible buffer to provide efficient primer annealing site for PCR Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction

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