4861480
Thermo Scientific™ ClaSeek™ Library Preparation Kit, Ion Torrent™ Compatible
Generate Illumina™-compatible next-gen sequencing libraries from low sample input using a one-tube PCR-free method with optimized master mixes
Manufacturer: Fischer Scientific
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Includes
ClaSeek End Conversion Master Mix IT, ClaSeek Ligation Mix IT, Adapters IT, Library Amplification Primer Mix, nuclease-free water, Cleanup Columns (pre-assembled with collection tubes)
Content And Storage
Store at -20°C. Cleanup Columns must be stored at room temperature.
For Use With (Application)
DNA fragment library preparation for downstream use in next generation sequencing workflow.
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Description
- Thermo Scientific ClaSeek Library Preparation Kit, Illumina™ compatible, is designed for fast and convenient construction of PCR-free NGS fragment library from DNA sample input as low as 500 ng
- In combination with the amplification protocol the kit also enables the generation of DNA fragment libraries from ultra-low 5 ng sample input
- The kit utilizes a highly efficient NGS-optimized library construction protocol combining end-conversion and adapter addition steps into a simple and convenient one-tube procedure
- This minimizes unnecessary pipetting steps and reduces hands-on time, allowing for PCR-free library construction in less than 70 minutes
- The kit is suitable for construction of 50, 300, 500 bp read-length Illumina™-compatible libraries and is validated for use on Illumina™ HiSeq™ and MiSeq™ sequencing platforms
- Highlights PCR-free - significantly minimized bias and improved coverage with amplification-free protocol Flexible - universal protocol for variable sample input (5 ng - 1 μg) and DNA fragment lengths Fast and Convenient - fewer cleanup steps, less hands-on time, optimized master mixes, one-tube end conversion procedure
- Applications DNA fragment library preparation for downstream use in next generation sequencing workflow
- Includes ClaSeek End Conversion Master Mix, IL ClaSeek Ligation Mix, IL Water, nuclease-free
SAFETY INFORMATION
- ShelfLife : 1 Year
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Includes: ClaSeek End Conversion Master Mix IT, ClaSeek Ligation Mix IT, Adapters IT, Library Amplification Primer Mix, nuclease-free water, Cleanup Columns (pre-assembled with collection tubes)
Content And Storage: Store at -20°C. Cleanup Columns must be stored at room temperature.
For Use With (Application): DNA fragment library preparation for downstream use in next generation sequencing workflow.
Includes:
ClaSeek End Conversion Master Mix IT, ClaSeek Ligation Mix IT, Adapters IT, Library Amplification Primer Mix, nuclease-free water, Cleanup Columns (pre-assembled with collection tubes)
Content And Storage:
Store at -20°C. Cleanup Columns must be stored at room temperature.
For Use With (Application):
DNA fragment library preparation for downstream use in next generation sequencing workflow.
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For Use With (Application): Genotyping, Hot-start PCR
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Genotyping, Hot-start PCR
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