Thermo Scientific™ ClaSeek Library Preparation Kit, Illumina compatible

Description

• Thermo Scientific ClaSeek Library Preparation Kit, Illumina™ compatible, is designed for fast and convenient construction of PCR-free NGS fragment library from DNA sample input as low as 500 ng
• In combination with the amplification protocol the kit also enables the generation of DNA fragment libraries from ultra-low 5 ng sample input
• The kit utilizes a highly efficient NGS-optimized library construction protocol combining end-conversion and adapter addition steps into a simple and convenient one-tube procedure
• This minimizes unnecessary pipetting steps and reduces hands-on time, allowing for PCR-free library construction in less than 70 minutes
• The kit is suitable for construction of 50, 300, 500 bp read-length Illumina™-compatible libraries and is validated for use on Illumina™ HiSeq™ and MiSeq™ sequencing platforms
• Highlights PCR-free - significantly minimized bias and improved coverage with amplification-free protocol Flexible - universal protocol for variable sample input (5 ng - 1 μg) and DNA fragment lengths Fast and Convenient - fewer cleanup steps, less hands-on time, optimized master mixes, one-tube end conversion procedure
• Applications DNA fragment library preparation for downstream use in next generation sequencing workflow
• Includes ClaSeek End Conversion Master Mix, IL ClaSeek Ligation Mix, IL Water, nuclease-free