Invitrogen™ FirstChoice™ RLM-RACE Kit

Description

• Description The FirstChoice RLM-RACE Kit is a major improvement over the basic rapid amplification of cDNA ends (RACE) protocol
• The RLM-RACE procedure selects only full-length mRNA—no rRNA, tRNA or degraded RNA—and facilitates the cloning of sequences from the 5' ends of messages
• From RNA to PCR product in less than a day Yields single, specific product from rare transcripts Selects 5' and/or 3' ends of true messages Efficient— Al enzymatic reactions are optimized to ensure detection of even rarest mRNA Final product size: 7kb or less Optimal reaction temperature: 42°C Sample: RNA Rapid amplification of cDNA ends (5'-RACE) is polymerase chain reaction-based technique developed to facilitate cloning of sequence from the 5'-ends of messages Improvement to basic RACE protocol Selects full-length mRNA—no rRNA, tRNA or degraded mRNA Optimal reaction temperature: 42°C Cloning, PCR and Real-Time PCR, Reverse Transcription, cDNA Libraries and Library Construction Order Info Shipping Condition: Dry ice