Novus Biologicals™ TLR4 Inhibitor Peptide Set

Human, Mouse, Rat

VIPER: A TLR4 Inhibitory Peptide: 1mg (lyophilized) KYSFKLILAEYRRRRRRRRR (VIPER sequence: KYSFKLILAEY). Molecular weight: 2780.3CP7, Control Peptide: 1mg (lyophilized) RNTISGNIYSARRRRRRRRR (Control sequence: RNTISGNIYSA). Molecular weight: 2601

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

TLR4 Inhibitor Peptide Set

TLR4

White

The inhibitor is used in assays to inhibit TLR4 activation; see Figure 3 and also refer to Lysakova-Devine et al (2010) for examples. Optimal inhibitor concentrations should be established through titration and may vary between model systems. We recommend an initial titration from 0-30 uM for in vitro assays (Fig 3). Control concentrations should mirror inhibitor concentrations. Inhibitor and control should be preincubated with cells prior to ligand activation to allow sufficient time for the peptides to enter from the media into the cell. We typically preincubate with inhibitor and control peptides for 2 h prior to TLR4 activation with LPS (Fig 3); however, optimal preincubation times may vary between model systems. The TLR4/MD-2/CD14 stably transfected cell line is a useful positive control model system for studying inhibition of TLR4 activation by VIPER (Fig 3). SEAP is used as a readout assay in Figure 3 to measure TLR4 inhibition. A novel model system is shown in Figure 1 where TL

2 Vials

2780.3

Lyophilized Solid