Molecular Probes™ Fetal Hemoglobin mAb Test Kit, R-PE Conjugate

Description

• The Fetal Hemoglobin (HbF) Test is based on a monoclonal antibody directed to fetal hemoglobin (hemoglobin F)
• This antibody is conjugated with R-phycoerythrin (R-PE)
• R-PE (phycoerythrin) is a stable and highly soluble phycobiliprotein which provides maximal absorbance and fluorescence emission without susceptibility to internal or external fluorescence quenching, thus providing an exceptional quantum yields and molar extinction coefficients
• Use of R-PE in antibody conjugates results in probes with greatly enhanced detectability
• This reagent is intended for the identification and subsequent enumeration of fetal red blood cells
• Fetal cells are identified by the presence of fetal hemoglobin by a flow cytometric method
• The Fetal Hemoglobin Test may be used as an aid in identifying fetal-maternal hemorrhage and determining the need for immunoprophylaxis with immune globulin
• Antibody Specifications Description: Fetal Hemoglobin F (HbF), mouse IgG1 monoclonal Label: R-PE (phycoerythrin) Validated Application: Flow Cytometry Species Reactivity: Human Ex/Em and laser configuration: 496/578 nm, blue laser Clone ID: HbF-1 Clinical Relevance The Fetal Hemoglobin Test contains a monoclonal antibody directed to HbF that specifically recognizes fetal hemoglobin found in fetal erythrocytes
• The test permits the enumeration of fetal erythrocytes in the maternal circulation as a result of a fetal-maternal hemorrhage, or leakage, of fetal cells into the maternal circulation
• Hemorrhage of cells from an Rh + fetus into the circulation of an Rh - mother may result in the formation of Rh - reactive antibodies in the mother
• Rh hemolytic anemia in a subsequent Rh + fetus may result from placental transfer of antibodies formed in the mother to the fetus
• Although the volume of fetal erythrocytes found in the maternal circulation during pregnancy and immediately post-partum is reported to be small and of uncertain clinical significance in many cases, substantial hemorrhage may result from a number of causes including fetal or maternal trauma and placental defects
• Erythrocytes containing fetal hemoglobin may be found in individuals of any age, but with lower amounts of fetal hemoglobin compared to fetal red cells
• These cells have been termed F cells
• High levels of F cells may also exist in adults with a heterogeneous group of genetic disorders of uncertain etiology referred to as Hereditary Persistence of Fetal Hemoglobin
• Other clinical conditions causing significant levels of anemia may also result in elevated levels of F cells
• Several clinical conditions have been described that involve increased levels of F cells
• These conditions include hereditary anemic diseases such as sickle cell anemia and thalassemia major
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