Thermo Scientific™ ALN Disc

Description

• Differentiate Fusobacterium species from Bacteroides species using Thermo Scientific™ Remel™ ALN Kit
• ALN Kit is recommended for use in detection of the enzyme L-alanyl-L-alanyl aminopeptidase in bacterial cells
• Detection of this enzyme is a rapid, cost-effective procedure for screening cultures by presumptively placing organisms in the appropriate genus ( Bacteroides spp
• are ALN-positive, while Fusobacterium spp
• are ALN-negative)
• The extent of anaerobe identification is a concern to laboratories due to the time and expense involved with some methods 1,2
• Anaerobe isolates from sites of serious infections and normally sterile body sites should be definitively characterized
• However, presumptive grouping and preliminary identification based on rapid techniques can provide clinically relevant information
• Use ALN Kit for detection of the enzyme, L-alanyl-L-alanyl aminopeptidase in bacterial cells
• Detection of this enzyme is a rapid, cost-effective procedure for screening cultures by presumptively placing organisms in the appropriate genus
• Easy to store - No special storage required Easy to interpret - With visual inspection The ALN disk contains a substrate L-alanyl-L-alanyl- β-naphthylamide
• Aminopeptidase hydrolyzes this substrate to release β-naphthylamine
• Following the addition of the Cinnamaldehyde Reagent, β-naphthylamine reacts with ρ-dimethylaminocinnamaldehyde to form a red colored complex, indicating a positive result.