Thermo Scientific™ Hippurate Disk

Description

• Rapidly detect the hydrolysis of sodium hippurate by β-hemolytic streptococci, Gardnerella vaginalis , and Campylobacter jejuni using a reagent-impregnated Thermo Scientific™ Remel™ Hippurate Disk
• Hwang and Ederer employed the ninhydrin reagent in a rapid method to detect hippurate hydrolysis by the end product glycine 1
• Jolly later outlined minimal criteria for identification of G
• vaginalis including hippurate hydrolysis 2
• In 1989, Cacho et al
• further evaluated the use of the hippurate disk in the identification of Campylobacter spp 3
• Rapidly detect the hydrolysis of sodium hippurate by β-hemolytic streptococci, Gardnerella vaginalis , and Campylobacter jejuni using a reagent-impregnated Hippurate Disk
• The hippurate hydrolysis test determines the ability of Group B streptococci, as well as other bacteria, to enzymatically hydrolyze sodium hippurate 4,5
• Ready to use: Reagent-impregnated disk Rapid test: Results are obtained within 30 minutes
• Easy to identify: A condensation reaction occurs with hydrindantin, ammonia, and residual ninhydrin to produce the final purple colored complex for a positive reaction and light gray color for a negative reaction
• Hippuric acid is hydrolyzed by the enzyme hippuricase to glycine and benzoic acid
• Ninhydrin evokes a five-step reaction beginning with the deamination of glycine to form hydrindantin (a reduced form of ninhydrin), carbon dioxide, and ammonia
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.