Invitrogen™ ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K531000-Each-of-1 | In Stock | ₹ 3,01,599.00 |
K531000 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,01,599.00
GST (18%): ₹ 54,287.82
Total Price: ₹ 3,55,886.82
Product Type
Lentiviral Expression Kit
Quantity
1 kit
Content And Storage
TOPO™ TA Cloning Reagents (Box 1)• pLenti6.3⁄V5-TOPO™ (5-10 ng⁄μL) 20 μL, -20°C• pLenti6.3⁄V5-GW⁄lacZ Control vector (0.5 ug⁄μL) 20 μL, -20°C• 10X PCR Buffer 100 μL, -20°C• l dNTP Mix 10 μL, -20°C• Salt Solution 50 μL, -20°C• CMV Forward Primer (100 ng⁄μL) 20 μL, -20°C• V5 (C-term) Reverse Primer (100 ng⁄μL) 20 μL, -20°C• Control PCR Template (50 ng⁄μL) 10 μL, -20°C• Control PCR Primers (100 ng⁄μL) 10 μL each, -20°C• Sterile Water 1 mLOne Shot™ Stbl3™ Chemically CompetentE. coli(Box 2)• Stbl3™ Cells 21 x 50 μL, -80°C• pUC19 control DNA (10 pg⁄μL) 50 μL, -80°C• S.O.C. Medium 6 mL, -80°CSupport kit• ViraPower™ Packaging Mix 195 μg, -20°C• Lipofectamine™ 2000 0.75 mL, +4°C• Blasticidin 50 mg, -20°C• 293 FT cells (3 x 106⁄mL) 1 mL, liquid nitrogen
Product Line
HiPerform™, TOPO™, ViraPower™
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Description
- The ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit combines Invitrogen’s ViraPower™ HiPerform™ Lentiviral and TOPO™ technologies to facilitate quick PCR-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells
- The pLenti6.3⁄V5-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements
- Advantages Stable expression Long-term experiments Accurate titer of functional virus Typically provides 5 minute, high-efficiency TOPO™ PCR cloning in three simple steps Key Features HiPerform™ WPRE and cPPT elements CMV promoter V5 epitope tag at C terminus Blasticidin selection Kit Includes pLenti6.3⁄V5-TOPO™ TA Cloning™ Kit (K5315-20) ViraPower™ Bsd Lentiviral Support Kit (K4970-00) 293FT Cell Line (R700-07) For research use only
- Not intended for any therapeutic or diagnostic use.
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Product Type: Lentiviral Expression Kit
Quantity: 1 kit
Content And Storage: TOPO™ TA Cloning Reagents (Box 1)• pLenti6.3⁄V5-TOPO™ (5-10 ng⁄μL) 20 μL, -20°C• pLenti6.3⁄V5-GW⁄lacZ Control vector (0.5 ug⁄μL) 20 μL, -20°C• 10X PCR Buffer 100 μL, -20°C• l dNTP Mix 10 μL, -20°C• Salt Solution 50 μL, -20°C• CMV Forward Primer (100 ng⁄μL) 20 μL, -20°C• V5 (C-term) Reverse Primer (100 ng⁄μL) 20 μL, -20°C• Control PCR Template (50 ng⁄μL) 10 μL, -20°C• Control PCR Primers (100 ng⁄μL) 10 μL each, -20°C• Sterile Water 1 mLOne Shot™ Stbl3™ Chemically CompetentE. coli(Box 2)• Stbl3™ Cells 21 x 50 μL, -80°C• pUC19 control DNA (10 pg⁄μL) 50 μL, -80°C• S.O.C. Medium 6 mL, -80°CSupport kit• ViraPower™ Packaging Mix 195 μg, -20°C• Lipofectamine™ 2000 0.75 mL, +4°C• Blasticidin 50 mg, -20°C• 293 FT cells (3 x 106⁄mL) 1 mL, liquid nitrogen
Product Line: HiPerform™, TOPO™, ViraPower™
Product Type: Expression Kit
Quantity: 1 kit
Content And Storage: Vector kit• pLenti6.3/V5-DEST™ (150 ng/μL), 40 μL, store at -20°C• pLenti6.3/V5-GW/lacZ Control vector (0.5 μg/μL), 20 μL, store at -20°C• One Shot™ Stbl3™ Chemically CompetentE. coli, 21 x 50 μL, store at -80°C• pUC19 control DNA (10 pg/μL), 50 μL, store at -80°C• S.O.C. Medium, 6 mL, store at -80°CSupport kit• ViraPower™ Packaging Mix, 195 μg, store at -20°C• Lipofectamine™ 2000, 0.75 mL, store at 4°C• Blasticidin, 50 mg, store at -20°C• 293 FT cells (3 x 106cells/mL), 1 mL, store in liquid nitrogen
Product Line: Gateway™, HiPerform™, ViraPower™
Product Type: Lentiviral Expression Kit
Quantity: 1 Kit
Content And Storage: pLenti7.3/V5-DEST™ Gateway™ Vector Kit• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL) , 20 μlLStore at -20°C.ViraPower™ Lentiviral Support KitContains enough reagents for 20 transfections in 10 cm tissue culture dishes:• Lyophilized Packaging Mix, 195 μg, store at -20°C• Lipofectamine™ 2000, 0.75 mL, store at 4°COne Shot™ Stbl3™ chemically competentE. colibox• One Shot™ Competent Cells, 20 single-use aliquots of 50 μL• S.O.C. medium• pUC19 supercoiled plasmid controlStore at -80°C.293FT cell line• 3 x 106frozen cells in 1 mL of Freezing Medium, store in liquid nitrogen.
Product Line: FastTiter™, Gateway™, HiPerform™, ViraPower™
