K533000
Invitrogen™ ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K533000-Each-of-1 | In Stock | ₹ 2,61,813.60 |
K533000 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,61,813.60
GST (18%): ₹ 47,126.448
Total Price: ₹ 3,08,940.048
Content And Storage
Vector kit• pLenti6.3/V5-DEST™ (150 ng/μL), 40 μL, store at -20°C• pLenti6.3/V5-GW/lacZ Control vector (0.5 μg/μL), 20 μL, store at -20°C• One Shot™ Stbl3™ Chemically CompetentE. coli, 21 x 50 μL, store at -80°C• pUC19 control DNA (10 pg/μL), 50 μL, store at -80°C• S.O.C. Medium, 6 mL, store at -80°CSupport kit• ViraPower™ Packaging Mix, 195 μg, store at -20°C• Lipofectamine™ 2000, 0.75 mL, store at 4°C• Blasticidin, 50 mg, store at -20°C• 293 FT cells (3 x 106cells/mL), 1 mL, store in liquid nitrogen
Constitutive or Inducible System
Constitutive
Cell Type
293-FT
Promoter
CMV
For Use With (Equipment)
HiPerform™, TOPO ™ , ViraPower™
Key Functions
Viral Expression
Product Type
Expression Kit
Quantity
1 kit
Special Elements
WPRE, cPPT
Cloning Method
Gateway
Delivery Type
Lentiviral
Format
Kit
Vector
pLenti, TOPO-TA Vector
For Use With (Application)
Protein Expression
Product Line
Gateway™, HiPerform™, ViraPower™
Protein Tag
V5 Epitope Tag
Selection Agent (Eukaryotic)
Blasticidin
Related Products
Description
- The ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit
- This kit combines Invitrogen's™ ViraPower™ HiPerform™ Lentiviral and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells
- The pLenti6.3/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements
- Advantages Stable expression Long-term experiments Accurate titer of functional virus Flexible and versatile Gateway™ recombination cloning technology Key Features HiPerform™ WPRE and cPPT elements CMV promoter V5 epitope tag at C terminus Blasticidin selection Kit includes pLenti6.3/V5-DEST™ Gateway™ Vector Kit (V533-06) ViraPower™ Bsd Lentiviral Support Kit (K4970-00) 293FT Cell Line (R700-07)
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Content And Storage: Vector kit• pLenti6.3/V5-DEST™ (150 ng/μL), 40 μL, store at -20°C• pLenti6.3/V5-GW/lacZ Control vector (0.5 μg/μL), 20 μL, store at -20°C• One Shot™ Stbl3™ Chemically CompetentE. coli, 21 x 50 μL, store at -80°C• pUC19 control DNA (10 pg/μL), 50 μL, store at -80°C• S.O.C. Medium, 6 mL, store at -80°CSupport kit• ViraPower™ Packaging Mix, 195 μg, store at -20°C• Lipofectamine™ 2000, 0.75 mL, store at 4°C• Blasticidin, 50 mg, store at -20°C• 293 FT cells (3 x 106cells/mL), 1 mL, store in liquid nitrogen
Constitutive or Inducible System: Constitutive
Cell Type: 293-FT
Promoter: CMV
For Use With (Equipment): HiPerform™, TOPO ™ , ViraPower™
Key Functions: Viral Expression
Product Type: Expression Kit
Quantity: 1 kit
Special Elements: WPRE, cPPT
Cloning Method: Gateway
Delivery Type: Lentiviral
Format: Kit
Vector: pLenti, TOPO-TA Vector
For Use With (Application): Protein Expression
Product Line: Gateway™, HiPerform™, ViraPower™
Protein Tag: V5 Epitope Tag
Selection Agent (Eukaryotic): Blasticidin
Content And Storage:
Vector kit• pLenti6.3/V5-DEST™ (150 ng/μL), 40 μL, store at -20°C• pLenti6.3/V5-GW/lacZ Control vector (0.5 μg/μL), 20 μL, store at -20°C• One Shot™ Stbl3™ Chemically CompetentE. coli, 21 x 50 μL, store at -80°C• pUC19 control DNA (10 pg/μL), 50 μL, store at -80°C• S.O.C. Medium, 6 mL, store at -80°CSupport kit• ViraPower™ Packaging Mix, 195 μg, store at -20°C• Lipofectamine™ 2000, 0.75 mL, store at 4°C• Blasticidin, 50 mg, store at -20°C• 293 FT cells (3 x 106cells/mL), 1 mL, store in liquid nitrogen
Constitutive or Inducible System:
Constitutive
Cell Type:
293-FT
Promoter:
CMV
For Use With (Equipment):
HiPerform™, TOPO ™ , ViraPower™
Key Functions:
Viral Expression
Product Type:
Expression Kit
Quantity:
1 kit
Special Elements:
WPRE, cPPT
Cloning Method:
Gateway
Delivery Type:
Lentiviral
Format:
Kit
Vector:
pLenti, TOPO-TA Vector
For Use With (Application):
Protein Expression
Product Line:
Gateway™, HiPerform™, ViraPower™
Protein Tag:
V5 Epitope Tag
Selection Agent (Eukaryotic):
Blasticidin
Content And Storage: pLenti7.3/V5-DEST™ Gateway™ Vector Kit• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL) , 20 μlLStore at -20°C.ViraPower™ Lentiviral Support KitContains enough reagents for 20 transfections in 10 cm tissue culture dishes:• Lyophilized Packaging Mix, 195 μg, store at -20°C• Lipofectamine™ 2000, 0.75 mL, store at 4°COne Shot™ Stbl3™ chemically competentE. colibox• One Shot™ Competent Cells, 20 single-use aliquots of 50 μL• S.O.C. medium• pUC19 supercoiled plasmid controlStore at -80°C.293FT cell line• 3 x 106frozen cells in 1 mL of Freezing Medium, store in liquid nitrogen.
Constitutive or Inducible System: Constitutive
Cell Type: __
Promoter: CMV
For Use With (Equipment): __
Key Functions: __
Product Type: Lentiviral Expression Kit
Quantity: 1 Kit
Special Elements: WPRE, cPPT
Cloning Method: Gateway
Delivery Type: Lentiviral
Format: __
Vector: pLenti
For Use With (Application): Reporter Assays, Viral Expression
Product Line: FastTiter™, Gateway™, HiPerform™, ViraPower™
Protein Tag: V5 Epitope Tag
Selection Agent (Eukaryotic): None
Content And Storage:
pLenti7.3/V5-DEST™ Gateway™ Vector Kit• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL) , 20 μlLStore at -20°C.ViraPower™ Lentiviral Support KitContains enough reagents for 20 transfections in 10 cm tissue culture dishes:• Lyophilized Packaging Mix, 195 μg, store at -20°C• Lipofectamine™ 2000, 0.75 mL, store at 4°COne Shot™ Stbl3™ chemically competentE. colibox• One Shot™ Competent Cells, 20 single-use aliquots of 50 μL• S.O.C. medium• pUC19 supercoiled plasmid controlStore at -80°C.293FT cell line• 3 x 106frozen cells in 1 mL of Freezing Medium, store in liquid nitrogen.
Constitutive or Inducible System:
Constitutive
Cell Type:
__
Promoter:
CMV
For Use With (Equipment):
__
Key Functions:
__
Product Type:
Lentiviral Expression Kit
Quantity:
1 Kit
Special Elements:
WPRE, cPPT
Cloning Method:
Gateway
Delivery Type:
Lentiviral
Format:
__
Vector:
pLenti
For Use With (Application):
Reporter Assays, Viral Expression
Product Line:
FastTiter™, Gateway™, HiPerform™, ViraPower™
Protein Tag:
V5 Epitope Tag
Selection Agent (Eukaryotic):
None
Content And Storage: __
Constitutive or Inducible System: __
Cell Type: __
Promoter: __
For Use With (Equipment): __
Key Functions: __
Product Type: __
Quantity: __
Special Elements: __
Cloning Method: __
Delivery Type: __
Format: __
Vector: __
For Use With (Application): __
Product Line: __
Protein Tag: __
Selection Agent (Eukaryotic): __
Content And Storage:
__
Constitutive or Inducible System:
__
Cell Type:
__
Promoter:
__
For Use With (Equipment):
__
Key Functions:
__
Product Type:
__
Quantity:
__
Special Elements:
__
Cloning Method:
__
Delivery Type:
__
Format:
__
Vector:
__
For Use With (Application):
__
Product Line:
__
Protein Tag:
__
Selection Agent (Eukaryotic):
__
Content And Storage: __
Constitutive or Inducible System: __
Cell Type: __
Promoter: __
For Use With (Equipment): For 3-position linear solvent recovery system
Key Functions: __
Product Type: __
Quantity: __
Special Elements: __
Cloning Method: __
Delivery Type: __
Format: __
Vector: __
For Use With (Application): __
Product Line: __
Protein Tag: __
Selection Agent (Eukaryotic): __
Content And Storage:
__
Constitutive or Inducible System:
__
Cell Type:
__
Promoter:
__
For Use With (Equipment):
For 3-position linear solvent recovery system
Key Functions:
__
Product Type:
__
Quantity:
__
Special Elements:
__
Cloning Method:
__
Delivery Type:
__
Format:
__
Vector:
__
For Use With (Application):
__
Product Line:
__
Protein Tag:
__
Selection Agent (Eukaryotic):
__