K175001
Multi-CopyPichiaExpression Kit
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K175001-Each-of-1 | In Stock | ₹ 2,15,611.20 |
K175001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,15,611.20
GST (18%): ₹ 38,810.016
Total Price: ₹ 2,54,421.216
Antibiotic Resistance Bacterial
Ampicillin (AmpR), Gentamicin (GmR)
Content And Storage
The Multi-CopyPichiaExpression Kit includes 20 μg each of pPIC3.5K, pPIC9K, and pAO815 vectors,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, Spheroplast Transformation Kit, and 2 μg each 5´AOX1, 3´AOX1, and α-factor sequencing primers. The pP1C9K, pPIC3.5K, and pAO815 vectors are also available separately.Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored.
Cell Type
Yeast Cells
Promoter
AOX1
Vector
pPIC
Expression System
Yeast
Product Type
Expression Kit
Quantity
1 Kit
Bacterial or Yeast Strain
KM71
Cloning Method
Restriction Enzyme/MCS
Format
Kit
Species
P. pastoris
Expression Mechanism
Cell-Based Expression
For Use With (Application)
Protein Expression
Protein Tag
Untagged
Selection Agent (Eukaryotic)
Ampicillin (AmpR), Gentamicin (GmR)
Related Products
Description
- The Multi-Copy Pichia Expression Kit contains the pPIC3.5K, pPIC9K, and pAO815 vectors for production and selection of Pichia strains that contain more than one copy of the gene of interest In many cases, increased copy number has led to increased expression levels These vectors are also available separately pPIC9K and pPIC3.5K The pPIC9K and pPIC3.5K vectors carry the kanamycin resistance gene that confers resistance to Geneticin™ Reagent in Pichi a Spontaneous generation of multiple insertion events can be identified by resistance to increased levels of Geneticin™ Reagent Pichia transformants are selected on histidine-deficient medium and screened for their level of resistance to Geneticin™ Reagent The ability to grow in high concentrations of Geneticin™ indicates that multiple copies of the kanamycin resistance gene and the gene of interest are integrated into the genome The pPIC9K vector directs secretion of expressed proteins while proteins expressed from pPIC3.5K remain intracellular
- pAO815 pAO815 is a Pichia expression vector designed to clone multiple copies of a gene into a single vector The vector contains a Bgl II site upstream of the 5´ AOX1 gene and a unique Ba mH I site downstream of the 3´ AOX1 transcription termination (TT) signal Four steps are required to generate multiple copies of the gene of interest: 1
- The gene is cloned into the unique Ec oR I site in the vector
- 2
- The construct is digested with Ba mH I and Bgl II to release the “expression cassette” containing the AOX1 promoter, gene of interest, and 3´ AOX1 TT
- 3
- Concatemers of the expression cassette are generated by ligation in vitr o
- 4
- The multiple copies are inserted back into the pAO815 vector and transformed into Pichi a.
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Antibiotic Resistance Bacterial: Ampicillin (AmpR), Gentamicin (GmR)
Content And Storage: The Multi-CopyPichiaExpression Kit includes 20 μg each of pPIC3.5K, pPIC9K, and pAO815 vectors,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, Spheroplast Transformation Kit, and 2 μg each 5´AOX1, 3´AOX1, and α-factor sequencing primers. The pP1C9K, pPIC3.5K, and pAO815 vectors are also available separately.Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored.
Cell Type: Yeast Cells
Promoter: AOX1
Vector: pPIC
Expression System: Yeast
Product Type: Expression Kit
Quantity: 1 Kit
Bacterial or Yeast Strain: KM71
Cloning Method: Restriction Enzyme/MCS
Format: Kit
Species: P. pastoris
Expression Mechanism: Cell-Based Expression
For Use With (Application): Protein Expression
Protein Tag: Untagged
Selection Agent (Eukaryotic): Ampicillin (AmpR), Gentamicin (GmR)
Antibiotic Resistance Bacterial:
Ampicillin (AmpR), Gentamicin (GmR)
Content And Storage:
The Multi-CopyPichiaExpression Kit includes 20 μg each of pPIC3.5K, pPIC9K, and pAO815 vectors,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, Spheroplast Transformation Kit, and 2 μg each 5´AOX1, 3´AOX1, and α-factor sequencing primers. The pP1C9K, pPIC3.5K, and pAO815 vectors are also available separately.Store vectors and primers at -20°C. Store strains at room temperature. All components are guaranteed stable for 6 months when properly stored.
Cell Type:
Yeast Cells
Promoter:
AOX1
Vector:
pPIC
Expression System:
Yeast
Product Type:
Expression Kit
Quantity:
1 Kit
Bacterial or Yeast Strain:
KM71
Cloning Method:
Restriction Enzyme/MCS
Format:
Kit
Species:
P. pastoris
Expression Mechanism:
Cell-Based Expression
For Use With (Application):
Protein Expression
Protein Tag:
Untagged
Selection Agent (Eukaryotic):
Ampicillin (AmpR), Gentamicin (GmR)
Antibiotic Resistance Bacterial: __
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ AGTRL1-bla U2OS cells• Tango™ AGTRL1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ AGTRL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type: __
Promoter: __
Vector: __
Expression System: __
Product Type: __
Quantity: 8 x 106Cells
Bacterial or Yeast Strain: __
Cloning Method: __
Format: __
Species: __
Expression Mechanism: __
For Use With (Application): __
Protein Tag: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial:
__
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• Tango™ AGTRL1-bla U2OS cells• Tango™ AGTRL1-bla U2OS Assay Protocol• Certificate of AnalysisThe Tango™ AGTRL1-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type:
__
Promoter:
__
Vector:
__
Expression System:
__
Product Type:
__
Quantity:
8 x 106Cells
Bacterial or Yeast Strain:
__
Cloning Method:
__
Format:
__
Species:
__
Expression Mechanism:
__
For Use With (Application):
__
Protein Tag:
__
Selection Agent (Eukaryotic):
__
Antibiotic Resistance Bacterial: __
Content And Storage: Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ EDG6-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70μg• Solution D• Tango™ EDG6-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ EDG6-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type: __
Promoter: __
Vector: __
Expression System: __
Product Type: __
Quantity: 8 x 106Cells
Bacterial or Yeast Strain: __
Cloning Method: __
Format: __
Species: __
Expression Mechanism: __
For Use With (Application): __
Protein Tag: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial:
__
Content And Storage:
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).Includes:• Tango™ EDG6-bla U2OS DA cells• LiveBLAzer™-FRET B/G Loading Kit, 70μg• Solution D• Tango™ EDG6-bla U2OS DA Assay Protocol• LiveBLAzer™-FRET B/G Loading Protocol• Certificate of AnalysisThe Tango™ EDG6-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type:
__
Promoter:
__
Vector:
__
Expression System:
__
Product Type:
__
Quantity:
8 x 106Cells
Bacterial or Yeast Strain:
__
Cloning Method:
__
Format:
__
Species:
__
Expression Mechanism:
__
For Use With (Application):
__
Protein Tag:
__
Selection Agent (Eukaryotic):
__
Antibiotic Resistance Bacterial: __
Content And Storage: K1761 Tango™ CCR6-bla U2OS cells(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).Includes:• Tango™ CCR6-bla U2OS cells• Tango™ CCR6-bla U2OS Assay Protocol• Certificate of AnalysisStorage:The Tango™ CCR6-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type: __
Promoter: __
Vector: __
Expression System: __
Product Type: __
Quantity: 2 x 106Cells
Bacterial or Yeast Strain: __
Cloning Method: __
Format: __
Species: __
Expression Mechanism: __
For Use With (Application): __
Protein Tag: __
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial:
__
Content And Storage:
K1761 Tango™ CCR6-bla U2OS cells(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).Includes:• Tango™ CCR6-bla U2OS cells• Tango™ CCR6-bla U2OS Assay Protocol• Certificate of AnalysisStorage:The Tango™ CCR6-bla U2OS cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type:
__
Promoter:
__
Vector:
__
Expression System:
__
Product Type:
__
Quantity:
2 x 106Cells
Bacterial or Yeast Strain:
__
Cloning Method:
__
Format:
__
Species:
__
Expression Mechanism:
__
For Use With (Application):
__
Protein Tag:
__
Selection Agent (Eukaryotic):
__