K171001
Invitrogen™PichiaExpression Kit, original kit
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K171001-Each-of-1 | In Stock | ₹ 2,23,835.00 |
K171001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,23,835.00
GST (18%): ₹ 40,290.30
Total Price: ₹ 2,64,125.30
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Content And Storage
The OriginalPichiaExpression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5'AOX1, 3'AOX1, and α-factor sequencing primers.Store vectors and primers at -20°C. Store strains at room temperature.All components are guaranteed stable for 6 months when properly stored.
Cell Type
Yeast Cells
Promoter
AOX1
Vector
pPIC
Expression System
Yeast
Product Type
Expression Kit
Quantity
1 Kit
Bacterial or Yeast Strain
GS115, KM71
Cloning Method
Restriction Enzyme⁄ MCS
Format
Kit
Species
P. pastoris
Expression Mechanism
Cell-Based Expression
For Use With (Application)
Protein Expression
Protein Tag
Untagged
Related Products
Description
- Description The Original Pichia Expression Kit is designed for high-level expression of recombinant protein in the yeast Pichia pastori s
- The vectors carry the HIS4 gene for selection of transformants on histidine-deficient medium
- The Original Pichia Expression Kit provides all of the tools and reagents needed to express a gene of interest from the AOX1 promoter.
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Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Content And Storage: The OriginalPichiaExpression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5'AOX1, 3'AOX1, and α-factor sequencing primers.Store vectors and primers at -20°C. Store strains at room temperature.All components are guaranteed stable for 6 months when properly stored.
Cell Type: Yeast Cells
Promoter: AOX1
Vector: pPIC
Expression System: Yeast
Product Type: Expression Kit
Quantity: 1 Kit
Bacterial or Yeast Strain: GS115, KM71
Cloning Method: Restriction Enzyme⁄ MCS
Format: Kit
Species: P. pastoris
Expression Mechanism: Cell-Based Expression
For Use With (Application): Protein Expression
Protein Tag: Untagged
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Content And Storage:
The OriginalPichiaExpression Kit includes 10 μg each of pHIL-D2 and pPIC3.5 vectors for intracellular expression and pHIL-S1 and pPIC9 vectors for secreted expression,Pichia pastorisstrains GS115(his4), KM71(arg4 his4 aox1::ARG4), control strain GS115 Albumin, control strain GS115 β-gal, media (YP and YNB), Spheroplast Transformation Kit, and 2 μg each 5'AOX1, 3'AOX1, and α-factor sequencing primers.Store vectors and primers at -20°C. Store strains at room temperature.All components are guaranteed stable for 6 months when properly stored.
Cell Type:
Yeast Cells
Promoter:
AOX1
Vector:
pPIC
Expression System:
Yeast
Product Type:
Expression Kit
Quantity:
1 Kit
Bacterial or Yeast Strain:
GS115, KM71
Cloning Method:
Restriction Enzyme⁄ MCS
Format:
Kit
Species:
P. pastoris
Expression Mechanism:
Cell-Based Expression
For Use With (Application):
Protein Expression
Protein Tag:
Untagged
Antibiotic Resistance Bacterial: __
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ 5HT1A Gα15-NFAT-blaCHO-K1 cells• GeneBLAzer™ 5HT1A Gα15-NFAT-blaCHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ 5HT1A Gα15 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type: __
Promoter: __
Vector: __
Expression System: __
Product Type: __
Quantity: 2 x 106Cells
Bacterial or Yeast Strain: __
Cloning Method: __
Format: __
Species: __
Expression Mechanism: __
For Use With (Application): FRET
Protein Tag: __
Antibiotic Resistance Bacterial:
__
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ 5HT1A Gα15-NFAT-blaCHO-K1 cells• GeneBLAzer™ 5HT1A Gα15-NFAT-blaCHO-K1 Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ 5HT1A Gα15 CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type:
__
Promoter:
__
Vector:
__
Expression System:
__
Product Type:
__
Quantity:
2 x 106Cells
Bacterial or Yeast Strain:
__
Cloning Method:
__
Format:
__
Species:
__
Expression Mechanism:
__
For Use With (Application):
FRET
Protein Tag:
__
Antibiotic Resistance Bacterial: __
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T cells• GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type: __
Promoter: __
Vector: __
Expression System: __
Product Type: __
Quantity: 2 x 106Cells
Bacterial or Yeast Strain: __
Cloning Method: __
Format: __
Species: __
Expression Mechanism: __
For Use With (Application): FRET
Protein Tag: __
Antibiotic Resistance Bacterial:
__
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T cells• GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ EDG3-Gα15-NFAT-blaHEK293T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type:
__
Promoter:
__
Vector:
__
Expression System:
__
Product Type:
__
Quantity:
2 x 106Cells
Bacterial or Yeast Strain:
__
Cloning Method:
__
Format:
__
Species:
__
Expression Mechanism:
__
For Use With (Application):
FRET
Protein Tag:
__
Antibiotic Resistance Bacterial: __
Content And Storage: LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ OPRL1-Gqi5-NFAT-blaFreestyle 293F cells• GeneBLAzer™ OPRL1-Gqi5-NFAT-blaFreestyle 293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ OPRL1-Gqi5-NFAT-blaFreestyle 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type: __
Promoter: __
Vector: __
Expression System: __
Product Type: __
Quantity: 2 x 106Cells
Bacterial or Yeast Strain: __
Cloning Method: __
Format: __
Species: __
Expression Mechanism: __
For Use With (Application): FRET
Protein Tag: __
Antibiotic Resistance Bacterial:
__
Content And Storage:
LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.Includes:• GeneBLAzer™ OPRL1-Gqi5-NFAT-blaFreestyle 293F cells• GeneBLAzer™ OPRL1-Gqi5-NFAT-blaFreestyle 293F Assay Protocol• Certificate of AnalysisThe GeneBLAzer™ OPRL1-Gqi5-NFAT-blaFreestyle 293F cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Cell Type:
__
Promoter:
__
Vector:
__
Expression System:
__
Product Type:
__
Quantity:
2 x 106Cells
Bacterial or Yeast Strain:
__
Cloning Method:
__
Format:
__
Species:
__
Expression Mechanism:
__
For Use With (Application):
FRET
Protein Tag:
__