A11141
Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A11141-Each-of-1 | In Stock | ₹ 4,35,500.40 |
A11141 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,35,500.40
GST (18%): ₹ 78,390.072
Total Price: ₹ 5,13,890.472
Antibiotic Resistance Bacterial
Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method
Gateway
Delivery Type
Lentiviral
Format
Kit
Vector
pLenti
For Use With (Equipment)
Gateway™, HiPerform™, T-REx™, ViraPower™
Inducing Agent
Doxycycline, Tetracycline
No. of Reactions
20 Reactions
Product Line
Gateway™, HiPerform™, T-REx™, ViraPower™
Protein Tag
V5 Epitope Tag
Replication Origin
pUC Origin
Selection Marker Promoter
PGK Promoter
Content And Storage
ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit(Cat. No. A11144)• pLenti6.3/TO/V5-DEST vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C, protect from light.Store at -20°C unless otherwise notedViraPower™ Bsd Lentiviral Support Kit(Cat. No. K497000)• ViraPower™ Lentiviral Packaging Mix (Contains 195 μL of a mixture of pLP1, pLP2, and pLP/VSVG plasmids, at 1 μg/μL in TE Buffer, pH 8.0), 195 μg. Store at -20°C.• Lipofectamine™ 2000 (Cat. No. 11668027), 0.75 mL. Store at 4°C (Do not freeze)• Blasticidin powder (Cat. No. R21001), 50 mg. Store at -20°C.293FT Cell Line(Cat. No. R70007)• 3 x 106frozen cells in 1 mL of Freezing Medium, 1 vial. Store in liquid nitrogenGateway™ LR Clonase™ II Plus Enzyme Mix(Cat. No. 12538120)• Gateway™ LR Clonase™ II Plus Enzyme Mix, 40 μL. Store -20°C for up to 6 months, -80°C for long term storage.• Proteinase K Solution (2 ug/mL), 40 μLOne Shot™ Stbl3™ Chemically Competent E. coli(Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL. Store at -80°C.Geneticin™(Cat. No. 10131035)• Geneticin™ solution (50 mg/mL), 20 mL, store at 4°C or -20°C• pENTR™ Gus positive control plasmid, store at -20°CpUC19 control DNA,(10 pg/μL), 50 μL
Constitutive or Inducible System
Inducible
Cell Type
293-FT
Promoter
CMV/TO
Gateway Enzyme
LR Clonase™ II Plus, LR Clonase™ II
For Use With (Application)
Protein Expression
Key Functions
Viral Expression
PCR Enzyme
Proof-Reading Polymerase
Product Type
Mammalian Expression system
Selection Agent (Eukaryotic)
Blasticidin
Selection Marker Eukaryotic
BsdR
Special Elements
WPRE, cPPT
Related Products
Description
- The ViraPower™ HiPerform™ T-REx™ Gateway™ Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit
- This kit combines Invitrogen's ViraPower™ HiPerform™ Lentiviral, T-REx™ and Gateway™ technologies to facilitate easy recombination-based cloning and lentiviral-based, regulated (Tetracycline-inducible), high-level expression of a target gene in dividing and non-dividing mammalian cells
- The pLenti6.3/ TO/V5-DEST vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements
- Advantages Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells Easy recombination-based cloning using Gateway™ technology Stable, long-term, tetracycline-regulated expression Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems Key Features WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, increases transgene expression and cPPT (central Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer
- WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements
- Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene Blasticidin selection marker for stable selection under control of SV40 promoter Kit includes ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit (Cat
- No
- A11144) ViraPower™ Bsd Lentiviral Support Kit (K497000) 293FT Cell Line (R70007) Gateway™ LR Clonase™ II Plus Enzyme Mix (12538120) One Shot™ Stbl3™ Chemically Competent E
- coli (C737303) Geneticin™ (10131035) pENTR™ Gus positive control plasmid
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Antibiotic Resistance Bacterial: Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method: Gateway
Delivery Type: Lentiviral
Format: Kit
Vector: pLenti
For Use With (Equipment): Gateway™, HiPerform™, T-REx™, ViraPower™
Inducing Agent: Doxycycline, Tetracycline
No. of Reactions: 20 Reactions
Product Line: Gateway™, HiPerform™, T-REx™, ViraPower™
Protein Tag: V5 Epitope Tag
Replication Origin: pUC Origin
Selection Marker Promoter: PGK Promoter
Content And Storage: ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit(Cat. No. A11144)• pLenti6.3/TO/V5-DEST vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C, protect from light.Store at -20°C unless otherwise notedViraPower™ Bsd Lentiviral Support Kit(Cat. No. K497000)• ViraPower™ Lentiviral Packaging Mix (Contains 195 μL of a mixture of pLP1, pLP2, and pLP/VSVG plasmids, at 1 μg/μL in TE Buffer, pH 8.0), 195 μg. Store at -20°C.• Lipofectamine™ 2000 (Cat. No. 11668027), 0.75 mL. Store at 4°C (Do not freeze)• Blasticidin powder (Cat. No. R21001), 50 mg. Store at -20°C.293FT Cell Line(Cat. No. R70007)• 3 x 106frozen cells in 1 mL of Freezing Medium, 1 vial. Store in liquid nitrogenGateway™ LR Clonase™ II Plus Enzyme Mix(Cat. No. 12538120)• Gateway™ LR Clonase™ II Plus Enzyme Mix, 40 μL. Store -20°C for up to 6 months, -80°C for long term storage.• Proteinase K Solution (2 ug/mL), 40 μLOne Shot™ Stbl3™ Chemically Competent E. coli(Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL. Store at -80°C.Geneticin™(Cat. No. 10131035)• Geneticin™ solution (50 mg/mL), 20 mL, store at 4°C or -20°C• pENTR™ Gus positive control plasmid, store at -20°CpUC19 control DNA,(10 pg/μL), 50 μL
Constitutive or Inducible System: Inducible
Cell Type: 293-FT
Promoter: CMV/TO
Gateway Enzyme: LR Clonase™ II Plus, LR Clonase™ II
For Use With (Application): Protein Expression
Key Functions: Viral Expression
PCR Enzyme: Proof-Reading Polymerase
Product Type: Mammalian Expression system
Selection Agent (Eukaryotic): Blasticidin
Selection Marker Eukaryotic: BsdR
Special Elements: WPRE, cPPT
Antibiotic Resistance Bacterial:
Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method:
Gateway
Delivery Type:
Lentiviral
Format:
Kit
Vector:
pLenti
For Use With (Equipment):
Gateway™, HiPerform™, T-REx™, ViraPower™
Inducing Agent:
Doxycycline, Tetracycline
No. of Reactions:
20 Reactions
Product Line:
Gateway™, HiPerform™, T-REx™, ViraPower™
Protein Tag:
V5 Epitope Tag
Replication Origin:
pUC Origin
Selection Marker Promoter:
PGK Promoter
Content And Storage:
ViraPower™ HiPerform™ T-Rex™ Gateway™ Vector Kit(Cat. No. A11144)• pLenti6.3/TO/V5-DEST vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C, protect from light.Store at -20°C unless otherwise notedViraPower™ Bsd Lentiviral Support Kit(Cat. No. K497000)• ViraPower™ Lentiviral Packaging Mix (Contains 195 μL of a mixture of pLP1, pLP2, and pLP/VSVG plasmids, at 1 μg/μL in TE Buffer, pH 8.0), 195 μg. Store at -20°C.• Lipofectamine™ 2000 (Cat. No. 11668027), 0.75 mL. Store at 4°C (Do not freeze)• Blasticidin powder (Cat. No. R21001), 50 mg. Store at -20°C.293FT Cell Line(Cat. No. R70007)• 3 x 106frozen cells in 1 mL of Freezing Medium, 1 vial. Store in liquid nitrogenGateway™ LR Clonase™ II Plus Enzyme Mix(Cat. No. 12538120)• Gateway™ LR Clonase™ II Plus Enzyme Mix, 40 μL. Store -20°C for up to 6 months, -80°C for long term storage.• Proteinase K Solution (2 ug/mL), 40 μLOne Shot™ Stbl3™ Chemically Competent E. coli(Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL. Store at -80°C.Geneticin™(Cat. No. 10131035)• Geneticin™ solution (50 mg/mL), 20 mL, store at 4°C or -20°C• pENTR™ Gus positive control plasmid, store at -20°CpUC19 control DNA,(10 pg/μL), 50 μL
Constitutive or Inducible System:
Inducible
Cell Type:
293-FT
Promoter:
CMV/TO
Gateway Enzyme:
LR Clonase™ II Plus, LR Clonase™ II
For Use With (Application):
Protein Expression
Key Functions:
Viral Expression
PCR Enzyme:
Proof-Reading Polymerase
Product Type:
Mammalian Expression system
Selection Agent (Eukaryotic):
Blasticidin
Selection Marker Eukaryotic:
BsdR
Special Elements:
WPRE, cPPT
Antibiotic Resistance Bacterial: Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method: Gateway
Delivery Type: Lentiviral
Format: __
Vector: pLenti
For Use With (Equipment): __
Inducing Agent: Doxycycline, Tetracycline
No. of Reactions: __
Product Line: Gateway™, HiPerform™, T-REx™, ViraPower™
Protein Tag: V5 Epitope Tag
Replication Origin: pUC Origin
Selection Marker Promoter: PGK Promoter
Content And Storage: ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit• pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.Store at -20°C unless otherwise notedOne Shot™ Stbl3™ Chemically CompetentE. coli(Cat. No. C737303)• Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C.
Constitutive or Inducible System: Inducible
Cell Type: __
Promoter: CMV/TO
Gateway Enzyme: LR Clonase™ II Plus, LR Clonase™ II
For Use With (Application): Viral Expression
Key Functions: __
PCR Enzyme: Proof-Reading Polymerase
Product Type: Vector Kit
Selection Agent (Eukaryotic): Blasticidin
Selection Marker Eukaryotic: BsdR
Special Elements: WPRE, cPPT
Antibiotic Resistance Bacterial:
Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method:
Gateway
Delivery Type:
Lentiviral
Format:
__
Vector:
pLenti
For Use With (Equipment):
__
Inducing Agent:
Doxycycline, Tetracycline
No. of Reactions:
__
Product Line:
Gateway™, HiPerform™, T-REx™, ViraPower™
Protein Tag:
V5 Epitope Tag
Replication Origin:
pUC Origin
Selection Marker Promoter:
PGK Promoter
Content And Storage:
ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit• pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.Store at -20°C unless otherwise notedOne Shot™ Stbl3™ Chemically CompetentE. coli(Cat. No. C737303)• Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C.
Constitutive or Inducible System:
Inducible
Cell Type:
__
Promoter:
CMV/TO
Gateway Enzyme:
LR Clonase™ II Plus, LR Clonase™ II
For Use With (Application):
Viral Expression
Key Functions:
__
PCR Enzyme:
Proof-Reading Polymerase
Product Type:
Vector Kit
Selection Agent (Eukaryotic):
Blasticidin
Selection Marker Eukaryotic:
BsdR
Special Elements:
WPRE, cPPT
Antibiotic Resistance Bacterial: Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method: Gateway
Delivery Type: Lentiviral
Format: __
Vector: pLenti
For Use With (Equipment): __
Inducing Agent: __
No. of Reactions: __
Product Line: Gateway™, HiPerform™, ViraPower™
Protein Tag: V5 Epitope Tag
Replication Origin: pUC Origin
Selection Marker Promoter: PGK Promoter
Content And Storage: pLenti6.4/R4R2/V5-DEST™ Kit• pLenti6.4/R4R2/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti6.4/CMV/V5-MSGW/lacZ plasmid (20 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/CMVp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/EF1αp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μgStore at -20°CpENTR 5' TOPO™ TA Cloning Kit(Cat. No. K95120)• pENTR 5' TOPO™ Reagents, store at -20°C.• One Shot™ TOP10 Chemically Competent E.coli (Cat. No. C404003), store at -80°C.One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C
Constitutive or Inducible System: Constitutive
Cell Type: __
Promoter: None (Promoterless), EF-1α, CMV
Gateway Enzyme: LR Clonase™ II Plus, LR Clonase™ II
For Use With (Application): Clone your own Promoter, Viral Expression
Key Functions: __
PCR Enzyme: Proof-Reading Polymerase
Product Type: Vector Kit
Selection Agent (Eukaryotic): Blasticidin
Selection Marker Eukaryotic: BsdR
Special Elements: WPRE, cPPT
Antibiotic Resistance Bacterial:
Ampicillin (AmpR), Blasticidin (BsdR)
Cloning Method:
Gateway
Delivery Type:
Lentiviral
Format:
__
Vector:
pLenti
For Use With (Equipment):
__
Inducing Agent:
__
No. of Reactions:
__
Product Line:
Gateway™, HiPerform™, ViraPower™
Protein Tag:
V5 Epitope Tag
Replication Origin:
pUC Origin
Selection Marker Promoter:
PGK Promoter
Content And Storage:
pLenti6.4/R4R2/V5-DEST™ Kit• pLenti6.4/R4R2/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti6.4/CMV/V5-MSGW/lacZ plasmid (20 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/CMVp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/EF1αp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μgStore at -20°CpENTR 5' TOPO™ TA Cloning Kit(Cat. No. K95120)• pENTR 5' TOPO™ Reagents, store at -20°C.• One Shot™ TOP10 Chemically Competent E.coli (Cat. No. C404003), store at -80°C.One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C
Constitutive or Inducible System:
Constitutive
Cell Type:
__
Promoter:
None (Promoterless), EF-1α, CMV
Gateway Enzyme:
LR Clonase™ II Plus, LR Clonase™ II
For Use With (Application):
Clone your own Promoter, Viral Expression
Key Functions:
__
PCR Enzyme:
Proof-Reading Polymerase
Product Type:
Vector Kit
Selection Agent (Eukaryotic):
Blasticidin
Selection Marker Eukaryotic:
BsdR
Special Elements:
WPRE, cPPT
Antibiotic Resistance Bacterial: __
Cloning Method: __
Delivery Type: __
Format: Kit
Vector: pPINK-LC, pPINK-HC
For Use With (Equipment): __
Inducing Agent: __
No. of Reactions: __
Product Line: __
Protein Tag: __
Replication Origin: __
Selection Marker Promoter: __
Content And Storage: The PichiaPink™ Secretion Optimization Kit consists of:• PichiaPink™ Vector kit containing a high- and a low copy number plasmid• PichiaPink™ Secretion Signal set containing 8 secretion signals• PichiaPink™ Expression Strain set containing 4 glycerol strains• PichiaPink™ Media Kit containing 4 basic mediaStore vectors and signal sequences at -20°CStore the PichiaPicha™ strains at -80°CStore the Media Kit at room temperature
Constitutive or Inducible System: __
Cell Type: Yeast Cells
Promoter: AOX1
Gateway Enzyme: __
For Use With (Application): Protein Expression
Key Functions: Secreted Expression
PCR Enzyme: __
Product Type: Optimization Kit
Selection Agent (Eukaryotic): __
Selection Marker Eukaryotic: __
Special Elements: __
Antibiotic Resistance Bacterial:
__
Cloning Method:
__
Delivery Type:
__
Format:
Kit
Vector:
pPINK-LC, pPINK-HC
For Use With (Equipment):
__
Inducing Agent:
__
No. of Reactions:
__
Product Line:
__
Protein Tag:
__
Replication Origin:
__
Selection Marker Promoter:
__
Content And Storage:
The PichiaPink™ Secretion Optimization Kit consists of:• PichiaPink™ Vector kit containing a high- and a low copy number plasmid• PichiaPink™ Secretion Signal set containing 8 secretion signals• PichiaPink™ Expression Strain set containing 4 glycerol strains• PichiaPink™ Media Kit containing 4 basic mediaStore vectors and signal sequences at -20°CStore the PichiaPicha™ strains at -80°CStore the Media Kit at room temperature
Constitutive or Inducible System:
__
Cell Type:
Yeast Cells
Promoter:
AOX1
Gateway Enzyme:
__
For Use With (Application):
Protein Expression
Key Functions:
Secreted Expression
PCR Enzyme:
__
Product Type:
Optimization Kit
Selection Agent (Eukaryotic):
__
Selection Marker Eukaryotic:
__
Special Elements:
__