A11144
Invitrogen™ ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A11144-Each-of-1 | In Stock | ₹ 3,30,261.60 |
A11144 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,30,261.60
GST (18%): ₹ 59,447.088
Total Price: ₹ 3,89,708.688
Constitutive or Inducible System
Inducible
Inducing Agent
Doxycycline, Tetracycline
Product Type
Vector Kit
Content And Storage
ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit• pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.Store at -20°C unless otherwise notedOne Shot™ Stbl3™ Chemically CompetentE. coli(Cat. No. C737303)• Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C.
Cleavage
No Cleavage Site
PCR Enzyme
Proof-Reading Polymerase
Replication Origin
pUC Origin
Selection Marker Eukaryotic
BsdR
Protein Tag
V5 Epitope Tag
Quantity
20 Reactions
Product Line
Gateway™, HiPerform™, T-REx™, ViraPower™
Delivery Type
Lentiviral
Promoter
CMV/TO
Selection Agent (Eukaryotic)
Blasticidin
Antibiotic Resistance Bacterial
Ampicillin (AmpR), Blasticidin (BsdR)
Gateway Enzyme
LR Clonase™ II Plus, LR Clonase™ II
Protein Tag Position (to your gene)
C-terminal
Selection Marker Promoter
PGK Promoter
Special Elements
WPRE, cPPT
Cloning Method
Gateway
Vector
pLenti
For Use With (Application)
Viral Expression
Related Products
Description
- The ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.3/TO/V5-DEST™ for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells
- The pLenti6.3/TO/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements
- Advantages Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells Easy recombination-based cloning using Gateway™ technology Stable, long-term, tetracycline-regulated expression Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems Key Features WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer
- WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements
- Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene Blasticidin selection marker for stable selection under control of SV40 promoter Kit includes pLenti6.3/TO/V5-DEST™ vector pLenti3.3/TR vector pLenti6.3/TO/V5-GW/lacZ plasmid Tetracycline One Shot™ Stbl3™ Chemically Competent E
- coli (Cat
- No
- C737303) pENTRTM Gus positive control plasmid.
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Constitutive or Inducible System: Inducible
Inducing Agent: Doxycycline, Tetracycline
Product Type: Vector Kit
Content And Storage: ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit• pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.Store at -20°C unless otherwise notedOne Shot™ Stbl3™ Chemically CompetentE. coli(Cat. No. C737303)• Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C.
Cleavage: No Cleavage Site
PCR Enzyme: Proof-Reading Polymerase
Replication Origin: pUC Origin
Selection Marker Eukaryotic: BsdR
Protein Tag: V5 Epitope Tag
Quantity: 20 Reactions
Product Line: Gateway™, HiPerform™, T-REx™, ViraPower™
Delivery Type: Lentiviral
Promoter: CMV/TO
Selection Agent (Eukaryotic): Blasticidin
Antibiotic Resistance Bacterial: Ampicillin (AmpR), Blasticidin (BsdR)
Gateway Enzyme: LR Clonase™ II Plus, LR Clonase™ II
Protein Tag Position (to your gene): C-terminal
Selection Marker Promoter: PGK Promoter
Special Elements: WPRE, cPPT
Cloning Method: Gateway
Vector: pLenti
For Use With (Application): Viral Expression
Constitutive or Inducible System:
Inducible
Inducing Agent:
Doxycycline, Tetracycline
Product Type:
Vector Kit
Content And Storage:
ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit• pLenti6.3/TO/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti3.3/TR vector (40 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 20 μg• pLenti6.3/TO/V5-GW/lacZ plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• Tetracycline (10 mg/mL in water), 1 mL. Store at -20°C and protect from light.Store at -20°C unless otherwise notedOne Shot™ Stbl3™ Chemically CompetentE. coli(Cat. No. C737303)• Stbl3™ Cells in 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C.
Cleavage:
No Cleavage Site
PCR Enzyme:
Proof-Reading Polymerase
Replication Origin:
pUC Origin
Selection Marker Eukaryotic:
BsdR
Protein Tag:
V5 Epitope Tag
Quantity:
20 Reactions
Product Line:
Gateway™, HiPerform™, T-REx™, ViraPower™
Delivery Type:
Lentiviral
Promoter:
CMV/TO
Selection Agent (Eukaryotic):
Blasticidin
Antibiotic Resistance Bacterial:
Ampicillin (AmpR), Blasticidin (BsdR)
Gateway Enzyme:
LR Clonase™ II Plus, LR Clonase™ II
Protein Tag Position (to your gene):
C-terminal
Selection Marker Promoter:
PGK Promoter
Special Elements:
WPRE, cPPT
Cloning Method:
Gateway
Vector:
pLenti
For Use With (Application):
Viral Expression
Constitutive or Inducible System: Constitutive
Inducing Agent: __
Product Type: Vector Kit
Content And Storage: pLenti6.4/R4R2/V5-DEST™ Kit• pLenti6.4/R4R2/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti6.4/CMV/V5-MSGW/lacZ plasmid (20 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/CMVp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/EF1αp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μgStore at -20°CpENTR 5' TOPO™ TA Cloning Kit(Cat. No. K95120)• pENTR 5' TOPO™ Reagents, store at -20°C.• One Shot™ TOP10 Chemically Competent E.coli (Cat. No. C404003), store at -80°C.One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C
Cleavage: No Cleavage Site
PCR Enzyme: Proof-Reading Polymerase
Replication Origin: pUC Origin
Selection Marker Eukaryotic: BsdR
Protein Tag: V5 Epitope Tag
Quantity: 20 Reactions
Product Line: Gateway™, HiPerform™, ViraPower™
Delivery Type: Lentiviral
Promoter: None (Promoterless), EF-1α, CMV
Selection Agent (Eukaryotic): Blasticidin
Antibiotic Resistance Bacterial: Ampicillin (AmpR), Blasticidin (BsdR)
Gateway Enzyme: LR Clonase™ II Plus, LR Clonase™ II
Protein Tag Position (to your gene): C-terminal
Selection Marker Promoter: PGK Promoter
Special Elements: WPRE, cPPT
Cloning Method: Gateway
Vector: pLenti
For Use With (Application): Clone your own Promoter, Viral Expression
Constitutive or Inducible System:
Constitutive
Inducing Agent:
__
Product Type:
Vector Kit
Content And Storage:
pLenti6.4/R4R2/V5-DEST™ Kit• pLenti6.4/R4R2/V5-DEST™ vector (40 μL of vector at 150 ng/μL in TE Buffer, pH 8.0), 6 μg• pLenti6.4/CMV/V5-MSGW/lacZ plasmid (20 μL of vector at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/CMVp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μg• pENTR 5'/EF1αp plasmid (20 μL of plasmid at 0.5 μg/μL in TE Buffer, pH 8.0), 10 μgStore at -20°CpENTR 5' TOPO™ TA Cloning Kit(Cat. No. K95120)• pENTR 5' TOPO™ Reagents, store at -20°C.• One Shot™ TOP10 Chemically Competent E.coli (Cat. No. C404003), store at -80°C.One Shot™ Stbl3™ Chemically Competent E. coli (Cat. No. C737303)• Stbl3™ Cells, 6 mL of S.O.C. Medium, 21 x 50 μL• pUC19 control DNA (10 pg/μL), 50 μLStore at -80°CpENTR™ Gus positive control plasmid, store at -20°C
Cleavage:
No Cleavage Site
PCR Enzyme:
Proof-Reading Polymerase
Replication Origin:
pUC Origin
Selection Marker Eukaryotic:
BsdR
Protein Tag:
V5 Epitope Tag
Quantity:
20 Reactions
Product Line:
Gateway™, HiPerform™, ViraPower™
Delivery Type:
Lentiviral
Promoter:
None (Promoterless), EF-1α, CMV
Selection Agent (Eukaryotic):
Blasticidin
Antibiotic Resistance Bacterial:
Ampicillin (AmpR), Blasticidin (BsdR)
Gateway Enzyme:
LR Clonase™ II Plus, LR Clonase™ II
Protein Tag Position (to your gene):
C-terminal
Selection Marker Promoter:
PGK Promoter
Special Elements:
WPRE, cPPT
Cloning Method:
Gateway
Vector:
pLenti
For Use With (Application):
Clone your own Promoter, Viral Expression
Constitutive or Inducible System: __
Inducing Agent: __
Product Type: Optimization Kit
Content And Storage: The PichiaPink™ Secretion Optimization Kit consists of:• PichiaPink™ Vector kit containing a high- and a low copy number plasmid• PichiaPink™ Secretion Signal set containing 8 secretion signals• PichiaPink™ Expression Strain set containing 4 glycerol strains• PichiaPink™ Media Kit containing 4 basic mediaStore vectors and signal sequences at -20°CStore the PichiaPicha™ strains at -80°CStore the Media Kit at room temperature
Cleavage: __
PCR Enzyme: __
Replication Origin: __
Selection Marker Eukaryotic: __
Protein Tag: __
Quantity: 1 Kit
Product Line: __
Delivery Type: __
Promoter: AOX1
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: __
Gateway Enzyme: __
Protein Tag Position (to your gene): __
Selection Marker Promoter: __
Special Elements: __
Cloning Method: __
Vector: pPINK-LC, pPINK-HC
For Use With (Application): Protein Expression
Constitutive or Inducible System:
__
Inducing Agent:
__
Product Type:
Optimization Kit
Content And Storage:
The PichiaPink™ Secretion Optimization Kit consists of:• PichiaPink™ Vector kit containing a high- and a low copy number plasmid• PichiaPink™ Secretion Signal set containing 8 secretion signals• PichiaPink™ Expression Strain set containing 4 glycerol strains• PichiaPink™ Media Kit containing 4 basic mediaStore vectors and signal sequences at -20°CStore the PichiaPicha™ strains at -80°CStore the Media Kit at room temperature
Cleavage:
__
PCR Enzyme:
__
Replication Origin:
__
Selection Marker Eukaryotic:
__
Protein Tag:
__
Quantity:
1 Kit
Product Line:
__
Delivery Type:
__
Promoter:
AOX1
Selection Agent (Eukaryotic):
__
Antibiotic Resistance Bacterial:
__
Gateway Enzyme:
__
Protein Tag Position (to your gene):
__
Selection Marker Promoter:
__
Special Elements:
__
Cloning Method:
__
Vector:
pPINK-LC, pPINK-HC
For Use With (Application):
Protein Expression
Constitutive or Inducible System: __
Inducing Agent: Methanol
Product Type: Vector Kit
Content And Storage: PichiaPink™ Secreted Protein Vector kit contains 20 μg pPinkα-HC (the high-copy number plasmid with the S. cerevisiae Alpha mating factor presequence) with sequencing primers 5' α-factor and 3' CYC1. Store at -20°C.
Cleavage: No Cleavage Site
PCR Enzyme: __
Replication Origin: pUC Origin
Selection Marker Eukaryotic: __
Protein Tag: Untagged
Quantity: __
Product Line: __
Delivery Type: __
Promoter: AOX1
Selection Agent (Eukaryotic): __
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Gateway Enzyme: __
Protein Tag Position (to your gene): __
Selection Marker Promoter: __
Special Elements: __
Cloning Method: Restriction Enzyme/MCS
Vector: pPink
For Use With (Application): __
Constitutive or Inducible System:
__
Inducing Agent:
Methanol
Product Type:
Vector Kit
Content And Storage:
PichiaPink™ Secreted Protein Vector kit contains 20 μg pPinkα-HC (the high-copy number plasmid with the S. cerevisiae Alpha mating factor presequence) with sequencing primers 5' α-factor and 3' CYC1. Store at -20°C.
Cleavage:
No Cleavage Site
PCR Enzyme:
__
Replication Origin:
pUC Origin
Selection Marker Eukaryotic:
__
Protein Tag:
Untagged
Quantity:
__
Product Line:
__
Delivery Type:
__
Promoter:
AOX1
Selection Agent (Eukaryotic):
__
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Gateway Enzyme:
__
Protein Tag Position (to your gene):
__
Selection Marker Promoter:
__
Special Elements:
__
Cloning Method:
Restriction Enzyme/MCS
Vector:
pPink
For Use With (Application):
__