Invitrogen™ ViraPower™ II Lentiviral Gateway™ Expression System
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K36720-Each-of-1 | In Stock | ₹ 2,44,273.80 |
K36720 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,44,273.80
GST (18%): ₹ 43,969.284
Total Price: ₹ 2,88,243.084
Constitutive or Inducible System
Constitutive
Promoter
CMV
Selection Agent (Eukaryotic)
Blasticidin
Protein Tag
V5 Epitope Tag
Quantity
1 kit
Product Line
Gateway™, ViraPower™
Delivery Type
Lentiviral
Product Type
Lentiviral Expression System
Content And Storage
pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)• One Shot™ Stbl3™ Chemically CompetentE. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)• 50 mg of Blasticidin powder (Cat # R21001)• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.Storage• Vectors: -20°C• ViraPower™ Packaging Mix: -20°C• Blasticidin: -20°C• Lipofectamine™ 2000: 4°C (do not freeze)• One Shot™ Stbl3™ Chemically CompetentE. coli:-80°C• 293 FT Cells: Liquid Nitrogen
Cloning Method
Gateway
Vector
pLenti
For Use With (Application)
Viral Expression
Related Products
Description
- Description Generates replication-incompetent lentivirus for transducing dividing and nondividing mammalian cells The pLenti6.2/V5-DESTvector in kit has CMV promoter for driving constitutive expression of target gene in mammalian cells Uses the PGK promoter instead of SV40 promoter in lentiviral vector for driving long-term, persistent expression of blasticidin stable selection marker Easy recombination-based cloning and stable expression, long term expression Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery
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Constitutive or Inducible System: Constitutive
Promoter: CMV
Selection Agent (Eukaryotic): Blasticidin
Protein Tag: V5 Epitope Tag
Quantity: 1 kit
Product Line: Gateway™, ViraPower™
Delivery Type: Lentiviral
Product Type: Lentiviral Expression System
Content And Storage: pLenti6.2⁄V5-DEST™ Gateway™ Vector Kit• 6 μg of pLenti6.2⁄V5-DEST™ vector (40 μLofvector at 150 ng⁄μL in TE Buffer, pH 8.0)• 10 μg of pLenti6.2⁄V5-GW⁄lacZ™ plasmid(20μL of plasmid at 0.5 μg⁄μL in TE Buffer, pH 8.0)• One Shot™ Stbl3™ Chemically CompetentE. coli(Cat # C737303): 21 x 50-μL of Stbl3™ Cells, 6 mL of S.O.C.Medium and 50 μL of pUC19 control DNA (10 pg⁄μL).ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)• 195 μg of ViraPower™ Packaging Mix (Contains 195μL of a mixture of pLP1, pLP2, and pLP⁄VSVG plasmids, at 1μg⁄μL in TE Buffer, pH 8.0)• 0.75 ml of Lipofectamine™ 2000 (Cat # 11668027)• 50 mg of Blasticidin powder (Cat # R21001)• 293FT Cell Line (Cat # R70007): 1 vial containing 3 X 106frozen cells in 1 mL of Freezing Medium.Storage• Vectors: -20°C• ViraPower™ Packaging Mix: -20°C• Blasticidin: -20°C• Lipofectamine™ 2000: 4°C (do not freeze)• One Shot™ Stbl3™ Chemically CompetentE. coli:-80°C• 293 FT Cells: Liquid Nitrogen
Cloning Method: Gateway
Vector: pLenti
For Use With (Application): Viral Expression
Constitutive or Inducible System: Inducible
Promoter: araBAD
Selection Agent (Eukaryotic): None
Protein Tag: His Tag (6x), V5 Epitope Tag, Thioredoxin
Quantity: 20 reactions
Product Line: TOPO™, ThioFusion™
Delivery Type: __
Product Type: Lentiviral Expression Kit
Content And Storage: Each pBAD/TOPO™ ThioFusion™ Expression Kit contains two boxes and the LMG194E. colistab. The pBAD/TOPO™ ThioFusion™ Expression box contains all of the reagents necessary for PCR (exceptTaqpolymerase) including 200 ng of topoisomerase I-activated pBAD/Thio-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers, 20% L-arabinose, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ box contains all of the reagents necessary for transformation including 21 single-use 50 μl aliquots of chemically competent TOP10E. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store the One Shot™ box at -80°C. Store the LMG194E. colistab at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.
Cloning Method: TOPO-TA
Vector: pBAD
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