V53406
Invitrogen™ pLenti7.3/V5-DEST™ Gateway™ Vector Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | V53406-Each-of-1 | In Stock | ₹ 2,30,955.00 |
V53406 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,30,955.00
GST (18%): ₹ 41,571.90
Total Price: ₹ 2,72,526.90
Constitutive or Inducible System
Constitutive
Promoter
CMV
Reporter Gene
GFP (EmGFP)
Content And Storage
pLenti7.3/V5-DEST™ Gateway™ Vector Kit• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL), 20 μLStore at -20°C.One Shot™ Stbl3™ Chemically CompetentE. coliboxTransformation reagents including:• One Shot™ Competent Cells, 20 x 50 μL single-use aliquots• S.O.C. medium• pUC19 supercoiled plasmid controlStore at -80°C.
Cloning Method
Gateway
Vector
pLenti
For Use With (Application)
Reporter Assays, Viral Expression
Delivery Type
Lentiviral
Product Type
Lentiviral Expression Vector
Selection Agent (Eukaryotic)
None
Protein Tag
V5 Epitope Tag
Quantity
1 Kit
Product Line
ViraPower™, Gateway™, HIPERFORM™
Related Products
Description
- The pLenti7.3/V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti7.3/V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells
- The pLenti7.3/V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements
- In addition, the vector allows for an accurate determination of titer of functional lentivirus in just two days using Emerald Green Fluorescent Protein (EmGFP)
- Advantages Transient expression Short-term experiments High-throughput screening 2 day titer of functional virus using EmGFP Flexible and versatile Gateway™ recombination cloning technology Key Features WPRE (Woodchuck Posttranscriptional Regulatory Element) from the woodchuck hepatitis virus, allows increased transgene expression and cPPT (Polypurine Tract) from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the gene of interest SV40 promoter driving expression of EmGFP Emerald Green Fluorescent Protein (EmGFP) allows easy determination of Lentiviral titers by flow cytometry and monitor transduced cells
- Kit Includes pLenti7.3/V5-DEST™ Gateway™ Vector box One Shot™ Stbl3™ chemically competent E
- coli (Cat
- No
- C7373-03) Related SKUs 293FT Cell Line (Cat
- No
- R70007) ViraPower™ Lentiviral Support Kit (K4970-00) ViraPower™ Lentiviral Packaging Mix (K4975-00) Lipofectamine™ 2000 (11668-019, 11668-027)
Compare Similar Items
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Constitutive or Inducible System: Constitutive
Promoter: CMV
Reporter Gene: GFP (EmGFP)
Content And Storage: pLenti7.3/V5-DEST™ Gateway™ Vector Kit• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL), 20 μLStore at -20°C.One Shot™ Stbl3™ Chemically CompetentE. coliboxTransformation reagents including:• One Shot™ Competent Cells, 20 x 50 μL single-use aliquots• S.O.C. medium• pUC19 supercoiled plasmid controlStore at -80°C.
Cloning Method: Gateway
Vector: pLenti
For Use With (Application): Reporter Assays, Viral Expression
Delivery Type: Lentiviral
Product Type: Lentiviral Expression Vector
Selection Agent (Eukaryotic): None
Protein Tag: V5 Epitope Tag
Quantity: 1 Kit
Product Line: ViraPower™, Gateway™, HIPERFORM™
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Reporter Gene:
GFP (EmGFP)
Content And Storage:
pLenti7.3/V5-DEST™ Gateway™ Vector Kit• pLenti7.3/V5-DEST™ Gateway™ Vector (150 ng/μL), 40 μL• pLenti7.3/V5-GW/lacZ Control Vector (0.5 μg/μL), 20 μLStore at -20°C.One Shot™ Stbl3™ Chemically CompetentE. coliboxTransformation reagents including:• One Shot™ Competent Cells, 20 x 50 μL single-use aliquots• S.O.C. medium• pUC19 supercoiled plasmid controlStore at -80°C.
Cloning Method:
Gateway
Vector:
pLenti
For Use With (Application):
Reporter Assays, Viral Expression
Delivery Type:
Lentiviral
Product Type:
Lentiviral Expression Vector
Selection Agent (Eukaryotic):
None
Protein Tag:
V5 Epitope Tag
Quantity:
1 Kit
Product Line:
ViraPower™, Gateway™, HIPERFORM™
Constitutive or Inducible System: __
Promoter: __
Reporter Gene: __
Content And Storage: __
Cloning Method: __
Vector: __
For Use With (Application): __
Delivery Type: __
Product Type: __
Selection Agent (Eukaryotic): __
Protein Tag: __
Quantity: __
Product Line: __
Constitutive or Inducible System:
__
Promoter:
__
Reporter Gene:
__
Content And Storage:
__
Cloning Method:
__
Vector:
__
For Use With (Application):
__
Delivery Type:
__
Product Type:
__
Selection Agent (Eukaryotic):
__
Protein Tag:
__
Quantity:
__
Product Line:
__
Constitutive or Inducible System: __
Promoter: __
Reporter Gene: __
Content And Storage: __
Cloning Method: __
Vector: __
For Use With (Application): __
Delivery Type: __
Product Type: __
Selection Agent (Eukaryotic): __
Protein Tag: __
Quantity: __
Product Line: __
Constitutive or Inducible System:
__
Promoter:
__
Reporter Gene:
__
Content And Storage:
__
Cloning Method:
__
Vector:
__
For Use With (Application):
__
Delivery Type:
__
Product Type:
__
Selection Agent (Eukaryotic):
__
Protein Tag:
__
Quantity:
__
Product Line:
__
Constitutive or Inducible System: Constitutive
Promoter: CMV
Reporter Gene: __
Content And Storage: Contains both pcDNA5⁄FRT and pcDNA5⁄FRT⁄CAT vectors. Store at -20°C
Cloning Method: Restriction Enzyme/MCS
Vector: pcDNA
For Use With (Application): Targeted Integration
Delivery Type: Transfection
Product Type: Mammalian Expression Vector
Selection Agent (Eukaryotic): Hygromycin
Protein Tag: Untagged
Quantity: 20 μg
Product Line: Flp-In™, pcDNA™
Constitutive or Inducible System:
Constitutive
Promoter:
CMV
Reporter Gene:
__
Content And Storage:
Contains both pcDNA5⁄FRT and pcDNA5⁄FRT⁄CAT vectors. Store at -20°C
Cloning Method:
Restriction Enzyme/MCS
Vector:
pcDNA
For Use With (Application):
Targeted Integration
Delivery Type:
Transfection
Product Type:
Mammalian Expression Vector
Selection Agent (Eukaryotic):
Hygromycin
Protein Tag:
Untagged
Quantity:
20 μg
Product Line:
Flp-In™, pcDNA™