Invitrogen™ pEF6/V5-His TOPO™ TA Expression Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K961020-Each-of-1 | In Stock | ₹ 1,01,645.28 |
K961020 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,01,645.28
GST (18%): ₹ 18,296.15
Total Price: ₹ 1,19,941.43
Constitutive or Inducible System
Constitutive
Promoter
EF-1α
Selection Agent (Eukaryotic)
Blasticidin
Cleavage
EK (Enterokinase) Recognition Site
Cloning Method
TOPO-TA
Vector
pEF, TOPO-TA Vectors
For Use With (Application)
Constitutive Expression
Delivery Type
Transfection
Product Type
TOPO TA Expression Kit
Content And Storage
Each pEF6/V5-His TOPO™ TA Expression Kit contains two boxes. The TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pEF6/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control templates and primers, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-μl aliquots of TOP10 Chemically CompetentE. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. Guaranteed stable for 6 months when properly stored.
Protein Tag
His Tag (6x), V5 Epitope Tag
Quantity
20 reactions
Product Line
TOPO™
Related Products
Description
- Enhancer/promoter elements from human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells Vector includes Blasticidin resistance gene for rapid selection of stable mammalian cell lines A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with Anti-His(C-term) Antibody Constitutive Expression, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis
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Constitutive or Inducible System: Constitutive
Promoter: EF-1α
Selection Agent (Eukaryotic): Blasticidin
Cleavage: EK (Enterokinase) Recognition Site
Cloning Method: TOPO-TA
Vector: pEF, TOPO-TA Vectors
For Use With (Application): Constitutive Expression
Delivery Type: Transfection
Product Type: TOPO TA Expression Kit
Content And Storage: Each pEF6/V5-His TOPO™ TA Expression Kit contains two boxes. The TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pEF6/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control templates and primers, primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Competent Cell box contains all of the reagents necessary for transformation, including twenty-one 50-μl aliquots of TOP10 Chemically CompetentE. coli, S.O.C. medium, and pUC19 supercoiled control plasmid. Store at -80°C. Guaranteed stable for 6 months when properly stored.
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 20 reactions
Product Line: TOPO™
Constitutive or Inducible System: __
Promoter: __
Selection Agent (Eukaryotic): __
Cleavage: __
Cloning Method: __
Vector: __
For Use With (Application): __
Delivery Type: __
Product Type: Lumio Expression and Detection System
Content And Storage: The Expressway™ Lumio™ Expression and Detection Systemincludes theE. coli slyD-Extract, 2.5X IVPSE. coliReaction Buffer, T7 Enzyme Mix, the Feed Buffer, 19 amino acid mix, Methionine, a control plasmid, DNAse/RNAse-free Water, and a Lumio™ Green Detection Kit for 20 real-time reactions or 100 in-gel applications. In addition to these components, the N-termExpressway™ Lumio™kit also includes 6 μg of the pEXP3-DEST vector and the C-termExpressway™ Lumio™kit 6 μg of the pEXP4-DEST vector. Store the vectors and the detection reagent at -20°C. Store all other components at -80°C. Guaranteed stable for 6 months when properly stored.
Protein Tag: __
Quantity: 1 kit
Product Line: Expressway™, Lumio™
Constitutive or Inducible System: __
Promoter: T7
Selection Agent (Eukaryotic): __
Cleavage: __
Cloning Method: __
Vector: __
For Use With (Application): __
Delivery Type: __
Product Type: Cell-Free Expression System
Content And Storage: __
Protein Tag: __
Quantity: 1 kit
Product Line: Expressway™